Structure and function of urea amidolyase
文献类型：期刊论文
| 作者 | Zhao, Jing2; Fan, Chen2; Xiang, Song2; Zhu, Li1; Wu, Yi1; , |
| 刊名 | BIOSCIENCE REPORTS
 |
| 出版日期 | 2018 |
| 卷号 | 38期号:-页码:BSR20171617 |
| 关键词 | Skin color Natural selection Genetic adaptation Modern humans Archaic hominin |
| ISSN号 | 0144-8463 |
| DOI | 10.1042/BSR20171617 |
| 文献子类 | Article |
| 英文摘要 | Urea is the degradation product of a wide range of nitrogen containing bio-molecules. Urea amidolyase (UA) catalyzes the conversion of urea to ammonium, the essential first step in utilizing urea as a nitrogen source. It is widely distributed in fungi, bacteria and other microorganisms, and plays an important role in nitrogen recycling in the biosphere. UA is composed of urea carboxylase (UC) and allophanate hydrolase (AH) domains, which catalyze sequential reactions. In some organisms UC and AH are encoded by separated genes. We present here structure of the Kluyveromyces lactis UA (KlUA). The structure revealed that KlUA forms a compact homo-dimer with a molecular weight of 400 kDa. Structure inspired biochemical experiments revealed the mechanism of its reaction intermediate translocation, and that the KlUA holo-enzyme formation is essential for its optimal activity. Interestingly, previous studies and ours suggest that UC and AH encoded by separated genes probably do not form a KlUA-like complex, consequently they might not catalyze the urea to ammonium conversion as efficiently. |
| 学科主题 | Biochemistry & Molecular Biology ; Cell Biology |
| WOS关键词 | ALLOPHANATE HYDROLASE ; ADENOSINE-TRIPHOSPHATE ; MICROBIAL UREASES ; PROVIDES INSIGHTS ; CARBOXYLASE ; PURIFICATION ; PATHWAY ; FAMILY ; DOMAIN ; FUNGI |
| 语种 | 英语 |
| WOS记录号 | WOS:000424695500014 |
| 出版者 | PORTLAND PRESS LTD |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/734]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所 |
| 作者单位 | 1.Lanzhou Univ, Sch Life Sci, Lanzhou 730000, Peoples R China, 2.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Nutr Sci, Key Lab Nutr & Metab, Shanghai 200031, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Zhao, Jing,Fan, Chen,Xiang, Song,et al. Structure and function of urea amidolyase[J]. BIOSCIENCE REPORTS,2018,38(-):BSR20171617. |
| APA | Zhao, Jing,Fan, Chen,Xiang, Song,Zhu, Li,Wu, Yi,&,.(2018).Structure and function of urea amidolyase.BIOSCIENCE REPORTS,38(-),BSR20171617. |
| MLA | Zhao, Jing,et al."Structure and function of urea amidolyase".BIOSCIENCE REPORTS 38.-(2018):BSR20171617. |
入库方式： OAI收割
来源：上海营养与健康研究所
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