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Genetic lineage tracing of resident stem cells by DeaLT

文献类型：期刊论文

作者He, Lingjuan2,3; Huang, Hefeng2; Li, Yan3; Huang, Xiuzhen3; Li, Yi3; Pu, Wenjuan3; Tian, Xueying3; Zhou, Bin3,4; Cai, Dongqing4; Lui, Kathy O.5
刊名NATURE PROTOCOLS
出版日期2018
卷号13期号:10页码:2217-2246
ISSN号1754-2189
DOI10.1038/s41596-018-0034-5
文献子类Article
英文摘要Unraveling the fates of resident stem cells during tissue regeneration is an important objective in clinical and basic research. Genetic lineage tracing based on Cre-loxP recombination provides an effective strategy for inferring cell fate and cell conversion in vivo. However, the determination of the exact fates of resident stem cells or their derivatives in disease states and during tissue regeneration remains controversial in many fields of study, partly because of technical limitations associated with Cre-based lineage tracing, such as, for example, off-target labeling. Recently, we generated a new lineage-tracing platform we named DeaLT (dual-recombinase-activated lineage tracing) that uses the Dre-rox recombination system to enhance the precision of Cre-mediated lineage tracing. Here, we describe as an example a detailed protocol using DeaLT to trace the fate of c-Kit(+) cardiac stem cells and their derivatives, in the absence of any interference from nontarget cells such as cardiomyocytes, during organ homeostasis and after tissue injury. This lineage-tracing protocol can also be used to delineate the fate of resident stem cells of other organ systems, and takes similar to 10 months to complete, from mouse crossing to final tissue analysis.
学科主题Biochemistry & Molecular Biology
WOS关键词TO-MESENCHYMAL TRANSITION ; C-KIT(+) CELLS ; MAMMARY-GLAND ; CARDIAC NEOVASCULARIZATION ; CORONARY-ARTERIES ; PROGENITOR CELLS ; ADULT PANCREAS ; BETA-CELLS ; CRE ; CONTRIBUTES
语种英语
出版者NATURE PUBLISHING GROUP
WOS记录号WOS:000446087000005
版本出版稿
源URL[http://202.127.25.144/handle/331004/744]  
专题中国科学院上海生命科学研究院营养科学研究所
作者单位1.Univ Chinese Acad Sci, Chinese Acad Sci, Shanghai Inst Biol Sci, Key Lab Nutr & Metab,Inst Nutr Sci, Shanghai, Peoples R China;
2.Shanghai Jiao Tong Univ, Int Peace Matern & Child Hlth Hosp, Sch Med, Shanghai, Peoples R China;
3.Univ Chinese Acad Sci, Chinese Acad Sci, CAS Ctr Excellence Mol Cell Sci, Shanghai Inst Biochem & Cell Biol,State Key Lab C, Shanghai, Peoples R China;
4.Jinan Univ, Key Lab Regenerat Med, Minist Educ, Guangzhou, Guangdong, Peoples R China;
5.Chinese Univ Hong Kong, Prince Wales Hosp, Li Ka Shing Inst Hlth Sci, Dept Chem Pathol, Shatin, Hong Kong, Peoples R China;
6.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China,
推荐引用方式
GB/T 7714		 He, Lingjuan,Huang, Hefeng,Li, Yan,et al. Genetic lineage tracing of resident stem cells by DeaLT[J]. NATURE PROTOCOLS,2018,13(10):2217-2246.
APA He, Lingjuan.,Huang, Hefeng.,Li, Yan.,Huang, Xiuzhen.,Li, Yi.,...&,.(2018).Genetic lineage tracing of resident stem cells by DeaLT.NATURE PROTOCOLS,13(10),2217-2246.
MLA He, Lingjuan,et al."Genetic lineage tracing of resident stem cells by DeaLT".NATURE PROTOCOLS 13.10(2018):2217-2246.

入库方式： OAI收割

来源：上海营养与健康研究所

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