One-step generation of complete gene knockout mice and monkeys by CRISPR/Cas9-mediated gene editing with multiple sgRNAs
文献类型：期刊论文
| 作者 | Zuo, Erwei2; Cai, Yi-Jun2; Li, Kui2; Wang, Bang-An2; Liu, Zhen2; Liu, Jiwei2; Hu, Xinde2; Wei, Wei2; Huo, Xiaona2; Shi, Linyu2 |
| 刊名 | CELL RESEARCH
 |
| 出版日期 | 2017 |
| 卷号 | 27期号:7页码:933-945 |
| 关键词 | complete gene knockout CRISPR/Cas9 multiple sgRNAs |
| ISSN号 | 1001-0602 |
| DOI | 10.1038/cr.2017.81 |
| 文献子类 | Article |
| 英文摘要 | The CRISPR/Cas9 system is an efficient gene-editing method, but the majority of gene-edited animals showed mosaicism, with editing occurring only in a portion of cells. Here we show that single gene or multiple genes can be completely knocked out in mouse and monkey embryos by zygotic injection of Cas9 mRNA and multiple adjacent single-guide RNAs (spaced 10-200 bp apart) that target only a single key exon of each gene. Phenotypic analysis of F0 mice following targeted deletion of eight genes on the Y chromosome individually demonstrated the robustness of this approach in generating knockout mice. Importantly, this approach delivers complete gene knockout at high efficiencies (100% on Arntl and 91% on Prrt2) in monkey embryos. Finally, we could generate a complete Prrt2 knockout monkey in a single step, demonstrating the usefulness of this approach in rapidly establishing gene-edited monkey models. |
| 学科主题 | Cell Biology |
| WOS关键词 | RNA-GUIDED ENDONUCLEASES ; OFF-TARGET MUTATIONS ; CYNOMOLGUS MONKEY ; MOUSE ; CAS9 ; CELLS ; TALEN ; CRISPR-CAS9 ; SPECIFICITY ; DISRUPTION |
| 语种 | 英语 |
| WOS记录号 | WOS:000404618500010 |
| 出版者 | INST BIOCHEMISTRY & CELL BIOLOGY |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/763]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所 |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Organ Chem, Interdisciplinary Res Ctr Biol & Chem, 345 Lingling Rd, Shanghai 200032, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Biol Sci, Institute Neurosci,State Key Lab Neurosci,Key Lab, CAS Ctr Excellence Brain Sci & Intelligence Techn, Shanghai 200031, Peoples R China; 3.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol,Shanghai Key Lab Mol And, CAS Ctr Excellence Mol Cell Sci,State Key Lab Cel, Shanghai 200031, Peoples R China; 4.Chinese Acad Sci, Univ Chinese Acad Sci, Shanghai Inst Biol Sci,State Key Lab Mol Biol, CAS Ctr Excellence Mol Cell Sci,Inst Biochem & Ce, Shanghai 200031, Peoples R China; 5.Shanghai Univ, Shanghai 200444, Peoples R China; 6.Guangxi Univ, Coll Anim Sci & Technol, Nanning 530004, Guangxi, Peoples R China; 7.Chinese Acad Sci, Shanghai Inst Biol Sci, CAS MPG Partner Inst Computat Biol, Key Lab Computat Biol, Shanghai 200031, Peoples R China; 8.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China, |
| 推荐引用方式 GB/T 7714 | Zuo, Erwei,Cai, Yi-Jun,Li, Kui,et al. One-step generation of complete gene knockout mice and monkeys by CRISPR/Cas9-mediated gene editing with multiple sgRNAs[J]. CELL RESEARCH,2017,27(7):933-945. |
| APA | Zuo, Erwei.,Cai, Yi-Jun.,Li, Kui.,Wang, Bang-An.,Liu, Zhen.,...&,.(2017).One-step generation of complete gene knockout mice and monkeys by CRISPR/Cas9-mediated gene editing with multiple sgRNAs.CELL RESEARCH,27(7),933-945. |
| MLA | Zuo, Erwei,et al."One-step generation of complete gene knockout mice and monkeys by CRISPR/Cas9-mediated gene editing with multiple sgRNAs".CELL RESEARCH 27.7(2017):933-945. |
入库方式： OAI收割
来源：上海营养与健康研究所
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