Deficiency in Fpr2 results in reduced numbers of Lin(-)cKit(+)Sca1(+) myeloid progenitor cells
文献类型：期刊论文
| 作者 | Chen, Keqiang4; Tang, Peng4; Bao, Zhiyao4; Gong, Wanghua4; Wang, Ji Ming4; Tessarollo, Lino5; Singh, Vijay K.6; Yoshimura, Teizo6; Tang, Peng7; Bao, Zhiyao8 |
| 刊名 | JOURNAL OF BIOLOGICAL CHEMISTRY
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| 出版日期 | 2018 |
| 卷号 | 293期号:35页码:13452-13463 |
| 关键词 | mouse bone marrow myeloid cell proliferation cytokine c-KIT Fpr2 Lin(-)c-Kit(+)Sca1(+)cells SCF |
| ISSN号 | 0021-9258 |
| DOI | 10.1074/jbc.RA118.002683 |
| 文献子类 | Article |
| 英文摘要 | The Lin(-)c-Kit(+) Sca-1(+) cell population in the bone marrow (BM) serves as the direct precursor for differentiation of myeloid cells. In this study, we report that deficiency in Fpr2, a G protein-coupled chemoattractant receptor in mice, is associated with reduced BM nucleated cells, including CD31(+)Ly6C(+) (granulocytes and monocytes), CD31(-)/Ly6C(int) (granuloid cells), and CD31(-)/Ly6C(high) (predominantly monocytes) cells. In particular, the number of Lin(-)c-Kit(+)Sca-1(+) (LKS) cells was reduced in Fpr2(-/-) mouse BM. This was supported by observations of the reduced incorporation of intraperitoneally injected bromodeoxyuridine by cells in the c-Kit(+) population from Fpr2(-/-) mouse BM. Purified c-Kit(+) cells from Fpr2(-/-) mice showed reduced expansion when cultured in vitro with stem cell factor (SCF). SCF/c-Kit-mediated phosphorylation of P38, STAT1, Akt (Thr-308), and Akt (Ser-473) was also significantly reduced in c-Kit(+) cells from Fpr2(-/-) mice. Furthermore, Fpr2 agonists enhanced SCF-induced proliferation of c-Kit(+) cells. Colony-forming unit assays revealed that CFU-granulocyte-macrophage formation of BM cells from Fpr2(-/-) mice was significantly reduced. After heat-inactivated bacterial stimulation in the airway, the expansion of c-kit(+) Sca-1(+) cells in BM and recruitment of Ly6G(+) cells to the lungs and CD11b(+)Ly6C(+)TNF(+) cells to the spleen of Fpr2(-/-) mice was significantly reduced. These results demonstrate an important role for Fpr2 in the development of myeloid lineage precursors in mouse BM. |
| 学科主题 | Biochemistry & Molecular Biology |
| WOS关键词 | HEMATOPOIETIC STEM-CELLS ; C-KIT LIGAND ; ESCHERICHIA-COLI BACTEREMIA ; FORMYL-PEPTIDE RECEPTORS ; BONE-MARROW ; FORMYLPEPTIDE RECEPTOR ; PERIPHERAL-BLOOD ; DENDRITIC CELLS ; LIPOXIN A(4) ; SELF-RENEWAL |
| 语种 | 英语 |
| WOS记录号 | WOS:000443375500009 |
| 出版者 | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/768]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所 |
| 作者单位 | 1.Leidos Biomed Res Inc, Basic Res Program, Frederick, MD 21702 USA; 2.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Nutr Sci, Shanghai 200031, Peoples R China, 3.Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Pathol & Expt Med, Okayama 7008558, Japan; 4.NCI, Canc & Inflammat Program, Ctr Canc Res, NIH, Frederick, MD 21702 USA; 5.NCI, Mouse Canc Genet Program, Ctr Canc Res, NIH, Frederick, MD 21702 USA; 6.Uniformed Serv Univ Hlth Sci, F Edward Hebert Sch Med, Armed Forces Radiobiol Res Inst, Dept Pharmacol & Mol Therapeut, Bethesda, MD 20814 USA; 7.Third Mil Med Univ, Southwest Hosp, Dept Breast Surg, Chongqing 400038, Peoples R China; 8.Shanghai Jiao Tong Univ, Sch Med, Ruijin Hosp, Dept Pulm & Crit Care Med, Shanghai 200025, Peoples R China; 9.Univ Macau, Inst Chinese Med Sci, State Key Lab Qual Res Chinese Med, Macau 999078, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Chen, Keqiang,Tang, Peng,Bao, Zhiyao,et al. Deficiency in Fpr2 results in reduced numbers of Lin(-)cKit(+)Sca1(+) myeloid progenitor cells[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2018,293(35):13452-13463. |
| APA | Chen, Keqiang.,Tang, Peng.,Bao, Zhiyao.,Gong, Wanghua.,Wang, Ji Ming.,...&,.(2018).Deficiency in Fpr2 results in reduced numbers of Lin(-)cKit(+)Sca1(+) myeloid progenitor cells.JOURNAL OF BIOLOGICAL CHEMISTRY,293(35),13452-13463. |
| MLA | Chen, Keqiang,et al."Deficiency in Fpr2 results in reduced numbers of Lin(-)cKit(+)Sca1(+) myeloid progenitor cells".JOURNAL OF BIOLOGICAL CHEMISTRY 293.35(2018):13452-13463. |
入库方式： OAI收割
来源：上海营养与健康研究所
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