Malignant ascites-derived organoid (MADO) cultures for gastric cancer in vitro modelling and drug screening
文献类型：期刊论文
| 作者 | Li, Jie4; Xu, Huawei4; Song, Lele4; Feng, Dan4; Peng, Xiaobo4; Wu, Meihong4; Wang, Bin4; Zhan, Xianbao4; Zhang, Lixing5; Zou, Yang1 |
| 刊名 | JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
 |
| 出版日期 | 2019 |
| 卷号 | 145期号:11页码:2637-2647 |
| 关键词 | Gastric cancer Malignant ascites Organoid Drug screening Personalised medicine |
| ISSN号 | 0171-5216 |
| DOI | 10.1007/s00432-019-03004-z |
| 文献子类 | Article |
| 英文摘要 | Purpose Malignant ascites (MA) is a common manifestation in advanced gastric cancer with peritoneal carcinomatosis and usually indicates a poor prognosis. However, lack of in vitro models that can faithfully recapitulate the characteristics of tumour cells in ascites hinders related researches. Tumour organoids have emerged as a robust in vitro model for tumour research and drug screening. Hence, we aimed to generate a 3-D in vitro organoid cultures from malignant ascites of gastric cancer for disease modelling and drug screening. Methods Eleven MADOs were generated from the MA tumour cells of gastric cancer patients. We made comparisons between MADOs and original MA tumour cells in histopathology by immunohistochemistry and genomics by whole-exome sequencing. In order to evaluate MADOs as functional in vitro disease models, we tested whether MADOs could be used for drug sensitivity screens. Results Eleven MADO cultures from human gastric cancer were established. MADOs demonstrated divergent growth characteristics and morphologies. MADO cultures preserve the histological architecture, genomic landscape of the corresponding MA tumour cells. MADOs exhibited heterogeneous responses to standard-of-care chemotherapeutics. Conclusions We generated MADOs modelling characteristics and mutated genes of MA tumour cells. A broad range of intrinsic MADO response to conventional chemotherapeutics suggests MADOs are amenable to drug screening. |
| 学科主题 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Genetics & Heredity |
| WOS关键词 | OVARIAN-CANCER ; CELL ; MUTATIONS ; CHEMOTHERAPY ; MANAGEMENT ; ADHESION ; BIOBANK |
| 语种 | 英语 |
| CSCD记录号 | CSCD:31598791 |
| WOS记录号 | WOS:000489287300001 |
| 出版者 | SPRINGER |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/770]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所 |
| 作者单位 | 1.East China Normal Univ, Sch Life Sci, Inst Biomed Sci, Shanghai Key Lab Regulatory Biol, Shanghai 200241, Peoples R China; 2.Fudan Univ, Shanghai Canc Ctr, Shanghai 230032, Peoples R China, 3.Fudan Univ, Inst Radiat Med, Shanghai 230032, Peoples R China; 4.Second Mil Med Univ, Changhai Hosp, Dept Oncol, Shanghai 200433, Peoples R China; 5.Shanghai 121Biomed Inc, Res & Early Dev, Shanghai 200235, Peoples R China; 6.Univ Chinese Acad Sci, CAS Key Lab Nutr Metab & Food Safety, Shanghai Inst Nutr & Hlth, Shanghai Inst Biol Sci,Chinese Acad Sci, Shanghai 200031, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Li, Jie,Xu, Huawei,Song, Lele,et al. Malignant ascites-derived organoid (MADO) cultures for gastric cancer in vitro modelling and drug screening[J]. JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY,2019,145(11):2637-2647. |
| APA | Li, Jie.,Xu, Huawei.,Song, Lele.,Feng, Dan.,Peng, Xiaobo.,...&,.(2019).Malignant ascites-derived organoid (MADO) cultures for gastric cancer in vitro modelling and drug screening.JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY,145(11),2637-2647. |
| MLA | Li, Jie,et al."Malignant ascites-derived organoid (MADO) cultures for gastric cancer in vitro modelling and drug screening".JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY 145.11(2019):2637-2647. |
入库方式： OAI收割
来源：上海营养与健康研究所
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