In vivo simultaneous transcriptional activation of multiple genes in the brain using CRISPR-dCas9-activator transgenic mice
文献类型：期刊论文
| 作者 | Zhou, Haibo1; Zhou, Changyang1,3; Gao, Ni1,3; Rao, Zhiping1,3; Li, He1,3; Hu, Xinde1,3; Li, Changlin1; Yao, Xuan1; Shen, Xiaowen1; Wei, Yu1 |
| 刊名 | NATURE NEUROSCIENCE
 |
| 出版日期 | 2018 |
| 卷号 | 21期号:3页码:440-+ |
| 关键词 | ancestral reconstruction copper odorant receptors orthologs sulfur |
| ISSN号 | 1097-6256 |
| DOI | 10.1038/s41593-017-0060-6 |
| 文献子类 | Article |
| 英文摘要 | Despite rapid progresses in the genome-editing field, in vivo simultaneous overexpression of multiple genes remains challenging. We generated a transgenic mouse using an improved dCas9 system that enables simultaneous and precise in vivo transcriptional activation of multiple genes and long noncoding RNAs in the nervous system. As proof of concept, we were able to use targeted activation of endogenous neurogenic genes in these transgenic mice to directly and efficiently convert astrocytes into functional neurons in vivo. This system provides a flexible and rapid screening platform for studying complex gene networks and gain-of-function phenotypes in the mammalian brain. |
| 学科主题 | Neurosciences & Neurology |
| WOS关键词 | CONVERTS FIBROBLASTS ; NEURONAL CELLS ; CRISPR ; GENOME ; EXPRESSION ; SYSTEM ; COMPLEX ; DIFFERENTIATION ; OVEREXPRESSION ; MODULATION |
| 语种 | 英语 |
| CSCD记录号 | CSCD:31835684 |
| WOS记录号 | WOS:000427776400019 |
| 出版者 | NATURE PUBLISHING GROUP |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/777]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所 |
| 作者单位 | 1.Chinese Acad Sci, CAS Ctr Excellence Brain Sci & Intelligence Techn, Shanghai Inst Biol Sci, Inst Neurosci,State Key Lab Neurosci, Shanghai, Peoples R China; 2.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China; 3.Univ Chinese Acad Sci, Coll Life Sci, Beijing, Peoples R China; 4.Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, Shanghai, Peoples R China; 5.Chinese Acad Sci, Shanghai Inst Biol Sci, CAS MPG Partner Inst Computat Biol, Key Lab Computat Biol, Shanghai, Peoples R China, |
| 推荐引用方式 GB/T 7714 | Zhou, Haibo,Zhou, Changyang,Gao, Ni,et al. In vivo simultaneous transcriptional activation of multiple genes in the brain using CRISPR-dCas9-activator transgenic mice[J]. NATURE NEUROSCIENCE,2018,21(3):440-+. |
| APA | Zhou, Haibo.,Zhou, Changyang.,Gao, Ni.,Rao, Zhiping.,Li, He.,...&,.(2018).In vivo simultaneous transcriptional activation of multiple genes in the brain using CRISPR-dCas9-activator transgenic mice.NATURE NEUROSCIENCE,21(3),440-+. |
| MLA | Zhou, Haibo,et al."In vivo simultaneous transcriptional activation of multiple genes in the brain using CRISPR-dCas9-activator transgenic mice".NATURE NEUROSCIENCE 21.3(2018):440-+. |
入库方式： OAI收割
来源：上海营养与健康研究所
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