CRISPR/Cas9-mediated targeted chromosome elimination
文献类型：期刊论文
| 作者 | Zuo, Erwei1; Huo, Xiaona1; Yao, Xuan1; Hu, Xinde1; He, Bingbing1; Wang, Xing1,5; Shi, Linyu1; Wei, Yu1; Ying, Wenqin1; Wei, Wei1 |
| 刊名 | GENOME BIOLOGY
 |
| 出版日期 | 2017 |
| 卷号 | 18期号:-页码:224 |
| 关键词 | Immune Regulation Tumor Immunosuppression microRNA15A/16-1 Colon Cancer Model |
| ISSN号 | 1474-760X |
| DOI | 10.1186/s13059-017-1354-4 |
| 文献子类 | Article |
| 英文摘要 | Background: The CRISPR/Cas9 system has become an efficient gene editing method for generating cells carrying precise gene mutations, including the rearrangement and deletion of chromosomal segments. However, whether an entire chromosome could be eliminated by this technology is still unknown. Results: Here we demonstrate the use of the CRISPR/Cas9 system to eliminate targeted chromosomes. Using either multiple cleavages induced by a single-guide RNA (sgRNA) that targets multiple chromosome-specific sites or a cocktail of multiple sgRNAs, each targeting one specific site, we found that a sex chromosome could be selectively eliminated in cultured cells, embryos, and tissues in vivo. Furthermore, this approach was able to produce a targeted autosome loss in aneuploid mouse embryonic stem cells with an extra human chromosome and human induced pluripotent stem cells with trisomy 21, as well as cancer cells. Conclusions: CRISPR/Cas9-mediated targeted chromosome elimination offers a new approach to develop animal models with chromosome deletions, and a potential therapeutic strategy for human aneuploidy diseases involving additional chromosomes. |
| 学科主题 | Biotechnology & Applied Microbiology ; Genetics & Heredity |
| WOS关键词 | PLURIPOTENT STEM-CELLS ; DOUBLE-STRANDED BREAKS ; MUSCULAR-DYSTROPHY ; TURNER-SYNDROME ; MOUSE MODEL ; GENOME ; MICE ; DNA ; GENERATION ; ANEUPLOIDY |
| 语种 | 英语 |
| WOS记录号 | WOS:000416043200001 |
| 出版者 | BIOMED CENTRAL LTD |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/797]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所 |
| 作者单位 | 1.Chinese Acad Sci, CAS Ctr Excellence Brain Sci & Intelligence Techn, Inst Neurosci,Shanghai Inst Biol Sci, State Key Lab Neurosci,Key Lab Primate Neurobiol, Shanghai 200031, Peoples R China; 2.Peking Univ, State Key Lab Membrane Biol, Sch Life Sci, Peking Tsinghua Ctr Life Sci, Beijing 100871, Peoples R China; 3.Peking Univ, Minstry Educ, Key Lab Cell Proliferat & Differentiat, Sch Life Sci,Peking Tsinghua Ctr Life Sci, Beijing 100871, Peoples R China; 4.Chinese Acad Sci, Key Lab Computat Biol, CAS MPG Partner Inst Computat Biol, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China; 5.Univ Chinese Acad Sci, Shanghai 200031, Peoples R China; 6.Shanghai Univ, Shanghai 200444, Peoples R China; 7.Vanderbilt Univ, Sch Med, Ctr Quantitat Sci, Nashville, TN 37232 USA; 8.Guangxi Univ, Coll Anim Sci & Technol, Nanning 530004, Guangxi, Peoples R China, |
| 推荐引用方式 GB/T 7714 | Zuo, Erwei,Huo, Xiaona,Yao, Xuan,et al. CRISPR/Cas9-mediated targeted chromosome elimination[J]. GENOME BIOLOGY,2017,18(-):224. |
| APA | Zuo, Erwei.,Huo, Xiaona.,Yao, Xuan.,Hu, Xinde.,He, Bingbing.,...&,.(2017).CRISPR/Cas9-mediated targeted chromosome elimination.GENOME BIOLOGY,18(-),224. |
| MLA | Zuo, Erwei,et al."CRISPR/Cas9-mediated targeted chromosome elimination".GENOME BIOLOGY 18.-(2017):224. |
入库方式： OAI收割
来源：上海营养与健康研究所
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