BRD4 interacts with PML/RAR in acute promyelocytic leukemia
文献类型：期刊论文
| 作者 | Luo, Qun1; Deng, Wanglong1; Fan, Huiyong1; Zhang, Ji1,2,3; Wang, Haiwei2,3; , |
| 刊名 | FRONTIERS OF MEDICINE
 |
| 出版日期 | 2018 |
| 卷号 | 12期号:6页码:726-734 |
| 关键词 | BRD4 PML RAR APL interaction |
| ISSN号 | 2095-0217 |
| DOI | 10.1007/s11684-017-0604-x |
| 文献子类 | Article |
| 英文摘要 | Bromodomain-containing 4 (BRD4) has been considered as an important requirement for disease maintenance and an attractive therapeutic target for cancer therapy. This protein can be targeted by JQ1, a selective small-molecule inhibitor. However, few studies have investigated whether BRD4 influenced acute promyelocytic leukemia (APL), and whether BRD4 had interaction with promyelocytic leukemia-retinoic acid receptor (PML/RAR) fusion protein to some extent. Results from cell viability assay, cell cycle analysis, and Annexin-V/PI analysis indicated that JQ1 inhibited the growth of NB4 cells, an APL-derived cell line, and induced NB4 cell cycle arrest at G1 and apoptosis. Then, we used co-immunoprecipitation (co-IP) assay and immunoblot to demonstrate the endogenous interaction of BRD4 and PML/RAR in NB4 cells. Moreover, downregulation of PML/RAR at the mRNA and protein levels was observed upon JQ1 treatment. Furthermore, results from the RT-qPCR, ChIP-qPCR, and re-ChIP-qPCR assays showed that BRD4 and PML/RAR co-existed on the same regulatory regions of their target genes. Hence, we showed a new discovery of the interaction of BRD4 and PML/RAR, as well as the decline of PML/RAR expression, under JQ1 treatment. |
| 学科主题 | Oncology ; Research & Experimental Medicine |
| WOS关键词 | P-TEFB ; SELECTIVE-INHIBITION ; CANCER ; RECRUITMENT ; EPIGENETICS ; ELONGATION ; MECHANISM ; SCREEN |
| 语种 | 英语 |
| WOS记录号 | WOS:000453348800013 |
| 出版者 | SPRINGER |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/798]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所 |
| 作者单位 | 1.Shanghai Jiao Tong Univ, Sch Med, Ruijin Hosp, State Key Lab Med Genom, Shanghai 200025, Peoples R China; 2.Chinese Acad Sci, Grad Sch, Shanghai 200025, Peoples R China, 3.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Hlth Sci, Shanghai 200025, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Luo, Qun,Deng, Wanglong,Fan, Huiyong,et al. BRD4 interacts with PML/RAR in acute promyelocytic leukemia[J]. FRONTIERS OF MEDICINE,2018,12(6):726-734. |
| APA | Luo, Qun,Deng, Wanglong,Fan, Huiyong,Zhang, Ji,Wang, Haiwei,&,.(2018).BRD4 interacts with PML/RAR in acute promyelocytic leukemia.FRONTIERS OF MEDICINE,12(6),726-734. |
| MLA | Luo, Qun,et al."BRD4 interacts with PML/RAR in acute promyelocytic leukemia".FRONTIERS OF MEDICINE 12.6(2018):726-734. |
入库方式： OAI收割
来源：上海营养与健康研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。