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MicroRNA 301A Promotes Intestinal Inflammation and Colitis-Associated Cancer Development by Inhibiting BTG1

文献类型：期刊论文

作者He, Chong2; Yu, Tianming2; Shi, Yan2; Ma, Caiyun2; Yang, Wenjing2; Fang, Leilei2; Sun, Mingming2; Wu, Wei2; Liu, Zhanju2; Xiao, Fei3
刊名GASTROENTEROLOGY
出版日期2017
卷号152期号:6页码:1434-+
关键词Mouse model Crohn's disease Ulcerative colitis Immune regulation
ISSN号0016-5085
DOI10.1053/j.gastro.2017.01.049
文献子类Article
英文摘要BACKGROUND & AIMS: Intestinal tissues from patients with inflammatory bowel disease (IBD) and colorectal cancer have increased expression of microRNA-301a (MIR301A) compared with tissues from patients without IBD. We studied the mechanisms of MIR301A in the progression of IBD in human tissues and mice. METHODS: We isolated intestinal epithelial cells (IECs) from biopsy samples of the colon from 153 patients with different stages of IBD activity, 6 patients with colitis-associated cancer (CAC), and 35 healthy individuals (controls), enrolled in the study in Shanghai, China. We measured expression of MIR301A and BTG anti-proliferation factor 1 (BTG1) by IECs using quantitative reverse-transcription polymerase chain reaction. Human colon cancer cell lines (HCT-116 and SW480) were transfected with a lentivirus that expresses MIR301A; expression of cytokines and tight junction proteins were measured by quantitative reverse transcription polymerase chain reaction, flow cytometry, and immunofluorescence staining. We generated mice with disruption of the microRNA-301A gene (MIR301A-knockout mice), and also studied mice that express a transgene-encoding BTG1. Colitis was induced in knockout, transgenic, and control (C57BL/B6) mice by administration of dextran sulfate sodium (DSS), and mice were given azoxymethane to induce colorectal carcinogenesis. Colons were collected and analyzed histologically and by immunohistochemistry; tumor nodules were counted and tumor size was measured. SW480 cells expressing the MIR301A transgene were grown as xenograft tumors in nude mice. RESULTS: Expression of MIR301A increased in IECs from patients with IBD and CAC compared with controls. MIR301A-knockout mice were resistant to the development of colitis following administration of DSS; their colon tissues expressed lower levels of interleukin 1 beta (IL1 beta), IL6, IL8, and tumor necrosis factor than colons of control mice. Colon tissues from MIR301A-knockout mice had increased epithelial barrier integrity and formed fewer tumors following administration of azoxymethane than control mice. Human IECs expressing transgenic MIR301A down-regulated expression of cadherin 1 (also called E-cadherin or CDH1). We identified BTG1 mRNA as a target of MIR301A; levels of BTG1 mRNA were reduced in inflamed mucosa from patients with active IBD compared with controls. There was an inverse correlation between levels of BTG1 mRNA and levels of MIR301A in inflamed mucosal tissues from patients with active IBD. Human colon cancer cell lines that expressed a MIR301A transgene increased proliferation; they had increased permeability and decreased expression of CDH1 compared with cells transfected with a control vector, indicating reduced intestinal barrier function. BTG1 transgenic mice developed less severe colitis than control mice following administration of DSS. SW480 cells expressing anti-MIR301A formed fewer xenograft tumors in nude mice than cells expressing a control vector. CONCLUSIONS: Levels of MIR301A are increased in IECs from patients with active IBD. MIR301A reduces expression of BTG1 to reduce epithelial integrity and promote inflammation in mouse colon and promotes tumorigenesis. Strategies to decrease levels of MIR301A in colon tissues might be developed to treat patients with IBD and CAC.
学科主题Gastroenterology & Hepatology
WOS关键词VITAMIN-D-RECEPTOR ; BOWEL-DISEASE ; TIGHT JUNCTION ; CELL-PROLIFERATION ; MUCOSAL BARRIER ; IMMUNE-SYSTEM ; TRANSLOCATION ; ACTIVATION ; MICROBIOTA ; APOPTOSIS
语种英语
WOS记录号WOS:000401811300036
出版者W B SAUNDERS CO-ELSEVIER INC
版本出版稿
源URL[http://202.127.25.144/handle/331004/807]  
专题中国科学院上海生命科学研究院营养科学研究所
作者单位1.Chinese Acad Med Sci, Peking Union Med Coll Hosp, Peking Union Med Coll, Dept Gastroenterol, Beijing, Peoples R China;
2.Tongji Univ, Shanghai Peoples Hosp 10, Dept Gastroenterol, Shanghai 200072, Peoples R China;
3.Chinese Acad Sci, Grad Sch, Shanghai Inst Biol Sci, Inst Nutr Sci,Key Lab Nutr & Metab, Shanghai, Peoples R China;
4.Sun Yat Sen Univ, Affiliated Hosp 1, Dept Gastroenterol, Guangzhou, Guangdong, Peoples R China;
5.Univ Texas Med Branch, Dept Immunol & Microbiol, Galveston, TX 77555 USA,
推荐引用方式
GB/T 7714		 He, Chong,Yu, Tianming,Shi, Yan,et al. MicroRNA 301A Promotes Intestinal Inflammation and Colitis-Associated Cancer Development by Inhibiting BTG1[J]. GASTROENTEROLOGY,2017,152(6):1434-+.
APA He, Chong.,Yu, Tianming.,Shi, Yan.,Ma, Caiyun.,Yang, Wenjing.,...&,.(2017).MicroRNA 301A Promotes Intestinal Inflammation and Colitis-Associated Cancer Development by Inhibiting BTG1.GASTROENTEROLOGY,152(6),1434-+.
MLA He, Chong,et al."MicroRNA 301A Promotes Intestinal Inflammation and Colitis-Associated Cancer Development by Inhibiting BTG1".GASTROENTEROLOGY 152.6(2017):1434-+.

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来源：上海营养与健康研究所

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