Simultaneous determination of amitraz, chlordimeform, formetanate and their main metabolites in human urine by high performance liquid chromatography-tandem mass spectrometry
文献类型：期刊论文
| 作者 | Gao, Xue2,3; Tan, Yanglan1; Guo, Hao4; , |
| 刊名 | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
 |
| 出版日期 | 2017 |
| 卷号 | 1052期号:-页码:27-33 |
| 关键词 | Formamidine pesticides Metabolites Human urine Solid supported liquid/liquid extraction (SLE) HPLC-MS/MS |
| ISSN号 | 1570-0232 |
| DOI | 10.1016/j.jchromb.2017.03.004 |
| 文献子类 | Article |
| 英文摘要 | A rapid, simple and reliable high performance liquid chromatography -tandem mass spectrometry (HPLC MS/MS) method was developed for simultaneous determination of amitraz, chlordimeform, formetanate and their main metabolites, N-(2,4-dimethylphenyl)-N-methyl-formamidine (DMPF), 2,4-dimethylformamidine (DMF), 2,4-dimethylaniline (DMA), 4-chloro-2-methylaniline and 3hydroxyacetanilide in human urine. The urine samples were mixed with buffer solutions (pH 8) and subsequently cleaned up by solid supported liquid/liquid extraction (SLE). The target analytes were efficiently separated with a Waters Atlantis T3 column (150 mm x 4.6 mm, 5 mu m), ionized with electrospray ion source in positive mode, and quantitatively determined by tandem mass spectrometry in the multiple reaction monitoring (MRM) mode. In order to minimize matrix effects, the matrix -matched calibration curves of eight analytes were adopted with correlation coefficients (R-2) above 0.99. The method were further validated by determining the limits of detection (LODs, 0.3-0.6 ng/mL), the limits of quantitation (LOQs, 1.0-2.0 ng/mL) and recoveries (89.1%-108.4%) with intra-day and inter -day relative standard deviation (RSD, <11%). The established method was applied and demonstrated in a real case by assaying a urine sample from a female poisoned by formetanate. The achieved results proved this method to be rapid, sensitive and accurate for simultaneous quantitation of eight analytes in human urine for intended forensic cases of human poisoning. (C) 2017 Elsevier B.V. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology ; Chemistry |
| WOS关键词 | SOLID-PHASE EXTRACTION ; AGRICULTURAL SAMPLES ; WHOLE-BLOOD ; LC-MS/MS ; PESTICIDE ; DEGRADATION ; FORMAMIDINE ; PLASMA ; IDENTIFICATION ; INSECTICIDES |
| 语种 | 英语 |
| WOS记录号 | WOS:000402218900004 |
| 出版者 | ELSEVIER SCIENCE BV |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/810]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所 |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Nutr Sci, Shanghai 200031, Peoples R China; 2.Chongqing Technol & Business Univ, Chongqing Key Lab Catalysis & Funct Organ Mol, Chongqing 400067, Peoples R China; 3.Chongqing Technol & Business Univ, Sch Resources & Environm, Chongqing 400067, Peoples R China; 4.Chongqing Inst Forens Sci, Chongqing 400021, Peoples R China, |
| 推荐引用方式 GB/T 7714 | Gao, Xue,Tan, Yanglan,Guo, Hao,et al. Simultaneous determination of amitraz, chlordimeform, formetanate and their main metabolites in human urine by high performance liquid chromatography-tandem mass spectrometry[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2017,1052(-):27-33. |
| APA | Gao, Xue,Tan, Yanglan,Guo, Hao,&,.(2017).Simultaneous determination of amitraz, chlordimeform, formetanate and their main metabolites in human urine by high performance liquid chromatography-tandem mass spectrometry.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1052(-),27-33. |
| MLA | Gao, Xue,et al."Simultaneous determination of amitraz, chlordimeform, formetanate and their main metabolites in human urine by high performance liquid chromatography-tandem mass spectrometry".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1052.-(2017):27-33. |
入库方式： OAI收割
来源：上海营养与健康研究所
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