Exosomal long non-coding RNA DLX6-AS1 as a potential diagnostic biomarker for non-small cell lung cancer
文献类型：期刊论文
| 作者 | Zhang, Xilin2; Guo, Huihui2; Bao, Ying2; Wang, Xiang2; Yu, Huanming1; Xie, Dong3; , |
| 刊名 | ONCOLOGY LETTERS
 |
| 出版日期 | 2019 |
| 卷号 | 18期号:5页码:5197-5204 |
| 关键词 | non-small cell lung cancer long non-coding RNA distal-less homeobox 6 antisense RNA 1 exosomes diagnosis |
| ISSN号 | 1792-1074 |
| DOI | 10.3892/ol.2019.10892 |
| 文献子类 | Article |
| 英文摘要 | Distal-less homeobox 6 antisense RNA 1 (DLX6-AS1) is upregulated in various solid tumors and serves a critical role in the tumorigenesis of cancer. However, to the best of our knowledge, the expression of circulating DLX6-AS1 and its role in the diagnosis of non-small cell lung cancer (NSCLC) have not been previously clarified. The aim of the present study was to investigate the expression and clinical significance of circulating DLX6-AS1 using reverse transcription-quantitative PCR in serum and exosomes derived from patients with NSCLC and healthy donors. The diagnostic value of circulating DLX6-AS1 was identified by receiver operating characteristic curve (ROC) analysis. First, it was revealed that the expression levels of DLX6-AS1 were significantly increased in tumor tissues compared with in adjacent normal tissues. In addition, DLX6-AS1 was highly expressed in NSCLC cell lines compared with in BEAS-2B cells. DLX6-AS1-knockdown inhibited cell proliferation and migration in vitro. It was subsequently demonstrated that the serum DLX6-AS1 level was significantly higher in patients with NSCLC compared with in healthy controls. Additionally, the higher DLX6-AS1 expression was associated with advanced disease stage, positive lymph node metastasis and poor tumor differentiation of NSCLC. ROC analysis demonstrated that the sensitivity and specificity of DLX6-AS1 were higher than those of CYFRA21-1, which is a serum marker for NSCLC. Finally, exosomal DLX6-AS1 expression was increased in patients with NSCLC compared with in healthy controls. The present data implied that circulating DLX6-AS1 was mainly incorporated into exosomes, providing a novel potential diagnostic marker for NSCLC. |
| 学科主题 | Biochemistry & Molecular Biology ; Mathematical & Computational Biology |
| WOS关键词 | EXPRESSION ; PROMOTES ; PROLIFERATION ; TUMORIGENESIS ; MIGRATION ; INVASION ; PATHWAY |
| 语种 | 英语 |
| CSCD记录号 | CSCD:31612030 |
| WOS记录号 | WOS:000503219600087 |
| 出版者 | SPANDIDOS PUBL LTD |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/817]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所 |
| 作者单位 | 1.First Peoples Hosp Huzhou, Dept Thorac Surg, Huzhou 313000, Zhejiang, Peoples R China; 2.Huzhou Univ, Affiliated Hosp 1, Cent Lab, Zhejiang Prov Key Lab Media Biol & Pathogen Contr, 158 Guangchang Back Rd, Huzhou 313000, Zhejiang, Peoples R China; 3.Chinese Acad Sci, Shanghai Inst Nutr & Hlth, Key Lab Nutr & Metab, 320 Yueyang Rd, Shanghai 200031, Peoples R China, |
| 推荐引用方式 GB/T 7714 | Zhang, Xilin,Guo, Huihui,Bao, Ying,et al. Exosomal long non-coding RNA DLX6-AS1 as a potential diagnostic biomarker for non-small cell lung cancer[J]. ONCOLOGY LETTERS,2019,18(5):5197-5204. |
| APA | Zhang, Xilin.,Guo, Huihui.,Bao, Ying.,Wang, Xiang.,Yu, Huanming.,...&,.(2019).Exosomal long non-coding RNA DLX6-AS1 as a potential diagnostic biomarker for non-small cell lung cancer.ONCOLOGY LETTERS,18(5),5197-5204. |
| MLA | Zhang, Xilin,et al."Exosomal long non-coding RNA DLX6-AS1 as a potential diagnostic biomarker for non-small cell lung cancer".ONCOLOGY LETTERS 18.5(2019):5197-5204. |
入库方式： OAI收割
来源：上海营养与健康研究所
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