Trio deep-sequencing does not reveal unexpected off-target and on-target mutations in Cas9-edited rhesus monkeys
文献类型：期刊论文
| 作者 | Luo, Xin2,3,4; He, Yaoxi2,3,4; He, Xiechao2,4; Li, Min2,3; Hu, Ting2,3; Hu, Yan2,3; Jiang, Jin2,3; Meng, Xiaoyu2,3; Zheng, Ping2; Su, Bing2,4,8 |
| 刊名 | NATURE COMMUNICATIONS
 |
| 出版日期 | 2019 |
| 卷号 | 10期号:-页码:5525 |
| 关键词 | cytokines inflammation macrophage monocytes preeclampsia |
| ISSN号 | 2041-1723 |
| DOI | 10.1038/s41467-019-13481-y |
| 文献子类 | Article |
| 英文摘要 | CRISPR-Cas9 is a widely-used genome editing tool, but its off-target effect and on-target complex mutations remain a concern, especially in view of future clinical applications. Non-human primates (NHPs) share close genetic and physiological similarities with humans, making them an ideal preclinical model for developing Cas9-based therapies. However, to our knowledge no comprehensive in vivo off-target and on-target assessment has been conducted in NHPs. Here, we perform whole genome trio sequencing of Cas9-treated rhesus monkeys. We only find a small number of de novo mutations that can be explained by expected spontaneous mutations, and no unexpected off-target mutations (OTMs) were detected. Furthermore, the long-read sequencing data does not detect large structural variants in the target region. |
| 学科主题 | Biochemistry & Molecular Biology ; Genetics & Heredity |
| WOS关键词 | READ ALIGNMENT ; PAIRED-END ; CRISPR-CAS9 ; INSERTIONS ; GENERATION ; FRAMEWORK ; MODEL |
| 语种 | 英语 |
| CSCD记录号 | CSCD:31797925 |
| WOS记录号 | WOS:000500505100001 |
| 出版者 | NATURE PUBLISHING GROUP |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/818]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所 |
| 作者单位 | 1.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China; 2.Chinese Acad Sci, Kunming Inst Zool, State Key Lab Genet Resources & Evolut, Kunming 650223, Yunnan, Peoples R China; 3.Univ Chinese Acad Sci, Kunming Coll Life Sci, Beijing 100101, Peoples R China; 4.Chinese Acad Sci, Kunming Primate Res Ctr, Kunming 650223, Yunnan, Peoples R China; 5.Chinese Acad Sci, CAS MPG Partner Inst Computat Biol PICB, Shanghai Inst Biol Sci, Key Lab Computat Biol,Max Planck Independent Res, Shanghai 200031, Peoples R China; 6.Chinese Acad Sci, Key Lab Anim Models & Human Dis Mech, Kunming Inst Zool, Kunming 650203, Yunnan, Peoples R China; 7.Kunming Univ Sci & Technol, Inst Primate Translat Med, Yunnan Key Lab Primate Biomed Res, Kunming 650500, Yunnan, Peoples R China; 8.Chinese Acad Sci, Ctr Excellence Anim Evolut & Genet, Kunming 650223, Yunnan, Peoples R China; 9.Collaborat Innovat Ctr Genet & Dev, Shanghai 200438, Peoples R China, |
| 推荐引用方式 GB/T 7714 | Luo, Xin,He, Yaoxi,He, Xiechao,et al. Trio deep-sequencing does not reveal unexpected off-target and on-target mutations in Cas9-edited rhesus monkeys[J]. NATURE COMMUNICATIONS,2019,10(-):5525. |
| APA | Luo, Xin.,He, Yaoxi.,He, Xiechao.,Li, Min.,Hu, Ting.,...&,.(2019).Trio deep-sequencing does not reveal unexpected off-target and on-target mutations in Cas9-edited rhesus monkeys.NATURE COMMUNICATIONS,10(-),5525. |
| MLA | Luo, Xin,et al."Trio deep-sequencing does not reveal unexpected off-target and on-target mutations in Cas9-edited rhesus monkeys".NATURE COMMUNICATIONS 10.-(2019):5525. |
入库方式： OAI收割
来源：上海营养与健康研究所
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