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SOX21 Ensures Rostral Forebrain Identity by Suppression of WNT8B during Neural Regionalization of Human Embryonic Stem Cells

文献类型：期刊论文

作者Fang, Zhuoqing2; Liu, Xinyuan2; Wen, Jing2; Zhou, Yang2; Jin, Ying2,3,5; Tang, Fan3; Jing, Naihe4,5; Zhou, Yang1,6; ,
刊名STEM CELL REPORTS
出版日期2019
卷号13期号:6页码:1038-1052
关键词Protein arginine methyltransferase 1 Hepatocellular carcinoma STAT3 signalling pathway Cryptotanshinone
ISSN号2213-6711
DOI10.1016/j.stemcr.2019.10.013
文献子类Article
英文摘要The generation of brain region-specific progenitors from human embryonic stem cells (hESCs) is critical for their application. However, transcriptional regulation of neural regionalization in humans is poorly understood. Here, we applied a rostrocaudal patterning system from hESCs to dissect global transcriptional networks controlling early neural regionalization. We found that SOX21 is required for rostral forebrain fate specification. SOX21 knockout led to activation of Wnt signaling, resulting in caudalization of regional identity of rostral forebrain neural progenitor cells. Moreover, we identified WNT8B as a SOX21 direct target. Deletion of WNT8B or inhibition of Wnt signaling in SOX21 knockout neural progenitor cells restored rostral forebrain identity. Furthermore, SOX21 interacted with beta-catenin, interfering with the binding of TCF4/beta-catenin complex to the WNT8B enhancer. Collectively, these results unveil the unknown role of SOX21 and shed light on how a transcriptional factor modulates early neural regionalization through crosstalk with a key component of Wnt signaling.
学科主题Cell Biology ; Physiology
WOS关键词HUMAN ES ; GENE-EXPRESSION ; SPECIFICATION ; REPRESSION ; NEURONS ; ANTEROPOSTERIOR ; DIFFERENTIATION ; PROGRESSION ; DISRUPTION ; PROTEINS
语种英语
CSCD记录号CSCD:31761677
WOS记录号WOS:000502098700008
出版者CELL PRESS
版本出版稿
源URL[http://202.127.25.144/handle/331004/819]  
专题中国科学院上海生命科学研究院营养科学研究所
作者单位1.Univ Alabama Birmingham, Sch Med, Dept Biomed Engn, Birmingham, AL 35294 USA;
2.Univ Chinese Acad Sci, CAS Key Lab Tissue Microenvironm & Tumor, CAS Ctr Excellence Mol Cell Sci,Chinese Acad Sci, Shanghai Inst Nutr & Hlth,Shanghai Inst Biol Sci, 320 Yueyang Rd, Shanghai 200031, Peoples R China;
3.Shanghai Jiao Tong Univ, Dept Histoembryol Genet & Dev Biol, Shanghai Key Lab Reprod Med, Basic Clin Res Ctr,Renji Hosp,Sch Med, 225 South Chongqing Rd, Shanghai 200025, Peoples R China;
4.Univ Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, CAS Ctr Excellence Mol Cell Sci, State Key Lab Cell Biol,Chinese Acad Sci, 320 Yue Yang Rd, Shanghai 200031, Peoples R China;
5.ShanghaiTech Univ, Sch Life Sci & Technol, 100 Haike Rd, Shanghai 201210, Peoples R China;
6.Univ Alabama Birmingham, Sch Engn, Birmingham, AL 35294 USA,
推荐引用方式
GB/T 7714		 Fang, Zhuoqing,Liu, Xinyuan,Wen, Jing,et al. SOX21 Ensures Rostral Forebrain Identity by Suppression of WNT8B during Neural Regionalization of Human Embryonic Stem Cells[J]. STEM CELL REPORTS,2019,13(6):1038-1052.
APA Fang, Zhuoqing.,Liu, Xinyuan.,Wen, Jing.,Zhou, Yang.,Jin, Ying.,...&,.(2019).SOX21 Ensures Rostral Forebrain Identity by Suppression of WNT8B during Neural Regionalization of Human Embryonic Stem Cells.STEM CELL REPORTS,13(6),1038-1052.
MLA Fang, Zhuoqing,et al."SOX21 Ensures Rostral Forebrain Identity by Suppression of WNT8B during Neural Regionalization of Human Embryonic Stem Cells".STEM CELL REPORTS 13.6(2019):1038-1052.

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来源：上海营养与健康研究所

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