PhoPepMass: A database and search tool assisting human phosphorylation peptide identification from mass spectrometry data
文献类型：期刊论文
| 作者 | Zhang, Menghuan1,2; Cui, Hui1,2; Xie, Lu2; Cui, Hui3; Chen, Lanming4; Yu, Ying5; Glocker, Michael O.6; , |
| 刊名 | JOURNAL OF GENETICS AND GENOMICS
 |
| 出版日期 | 2018 |
| 卷号 | 45期号:7页码:381-388 |
| 关键词 | Phosphosite Phosphopeptide Phosphopeptide mass |
| ISSN号 | 1673-8527 |
| DOI | 10.1016/j.jgg.2018.07.005 |
| 文献子类 | Article |
| 英文摘要 | Protein phosphorylation, one of the most important protein post-translational modifications, is involved in various biological processes, and the identification of phosphorylation peptides (phosphopeptides) and their corresponding phosphorylation sites (phosphosites) will facilitate the understanding of the molecular mechanism and function of phosphorylation. Mass spectrometry (MS) provides a high-throughput technology that enables the identification of large numbers of phosphosites. PhoPepMass is designed to assist human phosphopeptide identification from MS data based on a specific database of phophopeptide masses and a multivariate hypergeometric matching algorithm. It contains 244,915 phosphosites from several public sources. Moreover, the accurate masses of peptides and fragments with phosphosites were calculated. It is the first database that provides a systematic resource for the query of phosphosites on peptides and their corresponding masses. This allows researchers to search certain proteins of which phosphosites have been reported, to browse detailed phosphopeptide and fragment information, to match masses from MS analyses with defined threshold to the corresponding phosphopeptide, and to compare proprietary phosphopeptide discovery results with results from previous studies. Additionally, a database search software is created and a "two-stage search strategy" is suggested to identify phosphopeptides from tandem mass spectra of proteomics data. We expect PhoPepMass to be a useful tool and a source of reference for proteomics researchers. PhoPepMass is available at https://www.scbit.org/phopepmass/index. html. Copyright (C) 2018, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, and Genetics Society of China. Published by Elsevier Limited and Science Press. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology ; Genetics & Heredity |
| WOS关键词 | PROTEIN IDENTIFICATION ; TANDEM ; UPDATE ; SEQUENCES ; RESOURCE ; SPECTRA ; DEEP |
| 语种 | 英语 |
| WOS记录号 | WOS:000441805200006 |
| 出版者 | SCIENCE PRESS |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/849]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所 |
| 作者单位 | 1.Shanghai Tech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China; 2.Shanghai Acad Sci & Technol, Shanghai Ctr Bioinformat Technol, Shanghai 201203, Peoples R China; 3.Univ Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Nutr Sci, Shanghai 200031, Peoples R China; 4.Shanghai Ocean Univ, Coll Food Sci & Technol, Shanghai 201306, Peoples R China; 5.Tianjin Med Univ, Sch Basic Med Sci, Dept Pharmacol, Tianjin 30007, Peoples R China; 6.Univ Rostock, Proteome Ctr Rostock, D-18055 Rostock, Germany, |
| 推荐引用方式 GB/T 7714 | Zhang, Menghuan,Cui, Hui,Xie, Lu,et al. PhoPepMass: A database and search tool assisting human phosphorylation peptide identification from mass spectrometry data[J]. JOURNAL OF GENETICS AND GENOMICS,2018,45(7):381-388. |
| APA | Zhang, Menghuan.,Cui, Hui.,Xie, Lu.,Cui, Hui.,Chen, Lanming.,...&,.(2018).PhoPepMass: A database and search tool assisting human phosphorylation peptide identification from mass spectrometry data.JOURNAL OF GENETICS AND GENOMICS,45(7),381-388. |
| MLA | Zhang, Menghuan,et al."PhoPepMass: A database and search tool assisting human phosphorylation peptide identification from mass spectrometry data".JOURNAL OF GENETICS AND GENOMICS 45.7(2018):381-388. |
入库方式： OAI收割
来源：上海营养与健康研究所
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