Activation of G0S2 is coordinated by recruitment of PML/RAR alpha and C/EBP epsilon to its promoter during ATRA-induced APL differentiation
文献类型：期刊论文
| 作者 | Zhang, Fang2,4; Zhu, Yong Lan2,4; Deng, Wang Long2,4; Zhu, Jiang2,4; Zhang, Ji2,4; Zhang, Ji1,3; , |
| 刊名 | JOURNAL OF LEUKOCYTE BIOLOGY
 |
| 出版日期 | 2017 |
| 卷号 | 101期号:3页码:655-664 |
| 关键词 | all-trans retinoic acid acute promyelocytic leukemia neutrophil G0 G1 switch gene 2 |
| ISSN号 | 0741-5400 |
| DOI | 10.1189/jlb.1A0316-116R |
| 文献子类 | Article |
| 英文摘要 | All-trans retinoic acid (ATRA) binds the promyelocytic leukemia/retinoic acid receptor (PML/RAR) fusion protein and is an effective oncogene-targeted therapy for acute promyelocytic leukemia (APL). However, the molecular basis of PML/RAR-mediated transcriptional control during ATRA-induced differentiation is unclear. Previous studies have shown that the PML/RAR fusion protein behaves as a type II nuclear receptor, binding to DNA regardless of ligand status. Here, we performed a series of chromatin immunoprecipitation (ChIP)-quantitative PCR (qPCR) experiments, demonstrating that there is an additional mode of action of PML/RAR, wherein PML/RAR does not bind DNA in the absence of ATRA but binds DNA and activates adjacent genes in the presence of ATRA. This mode of action is similar to that of a type I nuclear receptor and is highlighted by activation of G0/G1 switch gene 2 (G0S2) during ATRA-induced neutrophil differentiation of leukemia cell lines (NB4 and PR9) and primary human APL cells. C/EBP epsilon occupancy of the G0S2 promoter was elevated in parallel with recruitment of PML/RAR in ATRA-treated NB4, PR9, and primary APL cells. Furthermore, we verified that the p30 isoform of C/EBP epsilon is crucial for activation of G0S2 and that PML/RAR interacts physically and cooperates functionally with C/EBP epsilon to up-regulate G0S2. Our data not only demonstrate a new mode of action of PML/RAR but also suggest a novel model in which PML/RAR synergizes with C/EBP epsilon to reactivate the C/EBP epsilon target G0S2, thereby contributing to ATRA-mediated APL differentiation and potentially, clinical remission. |
| 学科主题 | Cell Biology ; Hematology ; Immunology |
| WOS关键词 | ACUTE PROMYELOCYTIC LEUKEMIA ; ACUTE MYELOID-LEUKEMIA ; BINDING-PROTEIN EPSILON ; TRANS-RETINOIC ACID ; TRANSCRIPTION FACTOR ; GENE-EXPRESSION ; TARGET GENE ; TRANSLOCATION ; RECEPTOR ; CELLS |
| 语种 | 英语 |
| WOS记录号 | WOS:000395518100007 |
| 出版者 | FEDERATION AMER SOC EXP BIOL |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/882]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所 |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Hlth Sci, Shanghai, Peoples R China; 2.Shanghai Jiao Tong Univ, Sch Med, Ruijin Hosp, Shanghai Inst Hematol, Shanghai, Peoples R China; 3.Chinese Acad Sci, Grad Sch, Shanghai, Peoples R China, 4.Shanghai Jiao Tong Univ, Sch Med, Ruijin Hosp, State Key Lab Med Genom, Shanghai, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Zhang, Fang,Zhu, Yong Lan,Deng, Wang Long,et al. Activation of G0S2 is coordinated by recruitment of PML/RAR alpha and C/EBP epsilon to its promoter during ATRA-induced APL differentiation[J]. JOURNAL OF LEUKOCYTE BIOLOGY,2017,101(3):655-664. |
| APA | Zhang, Fang.,Zhu, Yong Lan.,Deng, Wang Long.,Zhu, Jiang.,Zhang, Ji.,...&,.(2017).Activation of G0S2 is coordinated by recruitment of PML/RAR alpha and C/EBP epsilon to its promoter during ATRA-induced APL differentiation.JOURNAL OF LEUKOCYTE BIOLOGY,101(3),655-664. |
| MLA | Zhang, Fang,et al."Activation of G0S2 is coordinated by recruitment of PML/RAR alpha and C/EBP epsilon to its promoter during ATRA-induced APL differentiation".JOURNAL OF LEUKOCYTE BIOLOGY 101.3(2017):655-664. |
入库方式： OAI收割
来源：上海营养与健康研究所
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