An ultrasensitive amperometric immunosensor for zearalenones based on oriented antibody immobilization on a glassy carbon electrode modified with MWCNTs and AuPt nanoparticles
文献类型：期刊论文
| 作者 | Liu, Na3; Tan, Yanglan3; Wang, Hui3; Wu, Aibo3; Nie, Dongxia4; Zhao, Zhiyong4; Liao, Yucai1; Sun, Changpo2; , |
| 刊名 | MICROCHIMICA ACTA
 |
| 出版日期 | 2017 |
| 卷号 | 184期号:1页码:147-153 |
| ISSN号 | 0026-3672 |
| 关键词 | Biosensor Electroanalysis Mycotoxins Electrodeposition Gold nanoparticles Platinum nanoparticles Cyclic voltammetry Differential pulse voltammetry Food analysis |
| DOI | 10.1007/s00604-016-1996-z |
| 文献子类 | Article |
| 英文摘要 | The family of zearalenones (ZENs) represents a major group of mycotoxins with estrogenic activity. They are produced by Fusarium fungi and cause adverse effects on human health and animal production. The authors describe here a label-free amperometric immunosensor for the direct determination of ZENs. A glassy carbon electrode (GCE) was first modified with polyethyleneimine-functionalized multi-walled carbon nanotubes. Next, gold and platinum nanoparticles (AuPt-NPs) were electro-deposited. This process strongly increased the surface area for capturing a large amount of antibodies and enhanced the electrochemical performance. In a final step, monoclonal antibody against zearalenone was orientedly immobilized on the electrode, this followed by surface blocking with BSA. The resulting biosensor was applied to the voltammetry determination of ZENs, best at a working voltage of 0.18 V (vs SCE). Under optimized conditions, the method displays a wide linear range that extends from 0.005 to 50 ng mL(-1), with a limit of detection of 1.5 pg mL(-1) (at an S/N ratio of 3). The assay is highly reproducible and selective, and therefore provides a sensitive and convenient tool for determination of such mycotoxins. |
| 学科主题 | Chemistry |
| WOS关键词 | ELECTROCHEMICAL IMMUNOSENSOR ; MASS-SPECTROMETRY ; PROTEIN-A ; CHROMATOGRAPHY ; FABRICATION ; NANOTUBES ; BIOSENSOR ; AMPLIFICATION ; IMMUNOASSAY ; MYCOTOXINS |
| 语种 | 英语 |
| 出版者 | SPRINGER WIEN |
| WOS记录号 | WOS:000389353900013 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/888]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所 |
| 作者单位 | 1.Huazhong Agr Univ, Coll Plant Sci & Technol, 1 Shizishan St, Wuhan 430070, Hubei, Peoples R China; 2.Acad State Adm Grain, 11 Baiwanzhuang Ave, Beijing 100037, Peoples R China, 3.Chinese Acad Sci, Shanghai Inst Biol Sci, Key Lab food safety Res Inst Nutr Sci, SIBS UGENT SJTU Joint Lab Mycotoxin Res, 294 Taiyuan Rd, Shanghai 200031, Peoples R China; 4.Shanghai Acad Agr Sci, Inst Agrifood Stand & Testing Technol, 1000 Jinqi Rd, Shanghai 201403, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Liu, Na,Tan, Yanglan,Wang, Hui,et al. An ultrasensitive amperometric immunosensor for zearalenones based on oriented antibody immobilization on a glassy carbon electrode modified with MWCNTs and AuPt nanoparticles[J]. MICROCHIMICA ACTA,2017,184(1):147-153. |
| APA | Liu, Na.,Tan, Yanglan.,Wang, Hui.,Wu, Aibo.,Nie, Dongxia.,...&,.(2017).An ultrasensitive amperometric immunosensor for zearalenones based on oriented antibody immobilization on a glassy carbon electrode modified with MWCNTs and AuPt nanoparticles.MICROCHIMICA ACTA,184(1),147-153. |
| MLA | Liu, Na,et al."An ultrasensitive amperometric immunosensor for zearalenones based on oriented antibody immobilization on a glassy carbon electrode modified with MWCNTs and AuPt nanoparticles".MICROCHIMICA ACTA 184.1(2017):147-153. |
入库方式： OAI收割
来源：上海营养与健康研究所
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