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ALYREF mainly binds to the 5 ' and the 3 ' regions of the mRNA in vivo

文献类型：期刊论文

作者Shi, Min2; Zhang, Heng2; Wang, Lantian2; Cheng, Hong2; Wu, Xudong5; Li, Guohui5; He, Zhisong1; Yin, Shanye4; Tian, Bin3; ,
刊名NUCLEIC ACIDS RESEARCH
出版日期2017
卷号45期号:16页码:9640-9653
关键词Brain-wide association study Random field theory Functional connectivity Statistical power
ISSN号0305-1048
DOI10.1093/nar/gkx597
文献子类Article
英文摘要The TREX complex (TREX) plays key roles in nuclear export of mRNAs. However, little is known about its transcriptome-wide binding targets. We used individual cross-linking and immunoprecipitation (iCLIP) to identify the binding sites of ALYREF, an mRNA export adaptor in TREX, in human cells. Consistent with previous in vitro studies, ALYREF binds to a region near the 5' end of the mRNA in a CBP80-dependent manner. Unexpectedly, we identified PABPN1-dependent ALYREF binding near the 3' end of the mRNA. Furthermore, the 3' processing factor CstF64 directly interacts with ALYREF and is required for the overall binding of ALYREF on the mRNA. In addition, we found that numerous middle exons harbor ALYREF binding sites and identified ALYREF-binding motifs that promote nuclear export of intronless mRNAs. Together, our study defines enrichment of ALYREF binding sites at the 5' and the 3' regions of the mRNA in vivo, identifies export-promoting ALYREF-binding motifs, and reveals CstF64-and PABPN1-mediated coupling of mRNA nuclear export to 3' processing.
学科主题Biochemistry & Molecular Biology
WOS关键词HUMAN TREX COMPLEX ; GENE-EXPRESSION ; NUCLEAR EXPORT ; COTRANSCRIPTIONAL RECRUITMENT ; SPLICING PROMOTES ; MRNP FORMATION ; THO COMPLEX ; INTRONLESS ; ELEMENT ; TRANSPORT
语种英语
WOS记录号WOS:000411096400037
出版者OXFORD UNIV PRESS
版本出版稿
源URL[http://202.127.25.144/handle/331004/893]  
专题中国科学院上海生命科学研究院营养科学研究所
作者单位1.Chinese Acad Sci, Shanghai Inst Biol Sci, CAS MPG Partner Inst Computat Biol, CAS Key Lab Computat Biol, Shanghai 200031, Peoples R China;
2.Chinese Acad Sci, Shanghai Inst Biol Sci, CAS Ctr Excellence Mol Cell Sci, Shanghai Key Lab Mol Androl,State Key Lab Mol Bio, Shanghai 200031, Peoples R China;
3.Rutgers New Jersey Med Sch, Dept Microbiol Biochem & Mol Genet, Newark, NJ 07103 USA,
4.Harvard Med Sch, Dept Cell Biol, Boston, MA 02115 USA;
5.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Mol Modeling & Design, State Key Lab Mol React Dynam, Dalian 116023, Peoples R China;
推荐引用方式
GB/T 7714		 Shi, Min,Zhang, Heng,Wang, Lantian,et al. ALYREF mainly binds to the 5 ' and the 3 ' regions of the mRNA in vivo[J]. NUCLEIC ACIDS RESEARCH,2017,45(16):9640-9653.
APA Shi, Min.,Zhang, Heng.,Wang, Lantian.,Cheng, Hong.,Wu, Xudong.,...&,.(2017).ALYREF mainly binds to the 5 ' and the 3 ' regions of the mRNA in vivo.NUCLEIC ACIDS RESEARCH,45(16),9640-9653.
MLA Shi, Min,et al."ALYREF mainly binds to the 5 ' and the 3 ' regions of the mRNA in vivo".NUCLEIC ACIDS RESEARCH 45.16(2017):9640-9653.

入库方式： OAI收割

来源：上海营养与健康研究所

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