An aldo-keto reductase is responsible for Fusarium toxin-degrading activity in a soil Sphingomonas strain
文献类型：期刊论文
| 作者 | He, Wei-Jie2,3; Yi, Shu-Yuan3; Tang, Xue-Ling3,5; Yuan, Qing-Song3,5; Guo, Mao-Wei3,5; Wu, Ai-Bo3; Qu, Bo3,5; Li, He-Ping2,3; Liao, Yu-Cai3,5; Zhang, Limin1 |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2017 |
| 卷号 | 7期号:-页码:9549 |
| 关键词 | HDL Apolipoprotein Glycation Proteomics Inflammation |
| ISSN号 | 2045-2322 |
| DOI | 10.1038/s41598-017-08799-w |
| 文献子类 | Article |
| 英文摘要 | Degradation of toxins by microorganisms is a promising approach for detoxification of agricultural products. Here, a bacterial strain, Sphingomonas S3-4, that has the ability to degrade the mycotoxin deoxynivalenol (DON) was isolated from wheat fields. Incubation of Fusarium-infected wheat grains with S3-4 completely eliminated DON. In S3-4 DON is catabolized into compounds with no detectable phytotoxicity, 3-oxo-DON and 3-epi-DON, via two sequential reactions. Comparative analysis of genome sequences from two DON-degrading strains, S3-4 and Devosia D17, and one non-DON-degrading strain, Sphingobium S26, combined with functional screening of a S3-4 genomic BAC library led to the discovery that a novel aldo/keto reductase superfamily member, AKR18A1, is responsible for oxidation of DON into 3-oxo-DON. DON-degrading activity is completely abolished in a mutant S3-4 strain where the AKR18A1 gene is disrupted. Recombinant AKR18A1 protein expressed in Escherichia coli catalyzed the reversible oxidation/reduction of DON at a wide range of pH values (7.5 to 11) and temperatures (10 to 50 degrees C). The S3-4 strain and recombinant AKR18A1 also catabolized zearalenone and the aldehydes glyoxal and methyglyoxal. The S3-4 strain and the AKR18A1 gene are promising agents for the control of Fusarium pathogens and detoxification of mycotoxins in plants and in food/feed products. |
| 学科主题 | Science & Technology - Other Topics |
| WOS关键词 | HEAD BLIGHT ; MYCOTOXIN DEOXYNIVALENOL ; ESCHERICHIA-COLI ; RNA GENES ; DETOXIFICATION ; WHEAT ; DEGRADATION ; RESISTANCE ; SUPERFAMILY ; ANNOTATION |
| 语种 | 英语 |
| WOS记录号 | WOS:000408449000028 |
| 出版者 | NATURE PUBLISHING GROUP |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/894]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所 |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Ctr Magnet Resonance, Wuhan Inst Phys & Math, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Peoples R China; 2.Huazhong Agr Univ, Coll Life Sci & Technol, Wuhan 430070, Peoples R China; 3.Huazhong Agr Univ, Mol Biotechnol Lab Triticeae Crops, Wuhan 430070, Peoples R China; 4.Huazhong Agr Univ, Natl Ctr Plant Gene Res Wuhan, Wuhan 430070, Peoples R China, 5.Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan 430070, Peoples R China; 6.Chinese Acad Sci, Shanghai Inst Biol Sci, Key Lab food safety, Res Inst Nutr Sci, Shanghai 200031, Peoples R China; |
| 推荐引用方式 GB/T 7714 | He, Wei-Jie,Yi, Shu-Yuan,Tang, Xue-Ling,et al. An aldo-keto reductase is responsible for Fusarium toxin-degrading activity in a soil Sphingomonas strain[J]. SCIENTIFIC REPORTS,2017,7(-):9549. |
| APA | He, Wei-Jie.,Yi, Shu-Yuan.,Tang, Xue-Ling.,Yuan, Qing-Song.,Guo, Mao-Wei.,...&,.(2017).An aldo-keto reductase is responsible for Fusarium toxin-degrading activity in a soil Sphingomonas strain.SCIENTIFIC REPORTS,7(-),9549. |
| MLA | He, Wei-Jie,et al."An aldo-keto reductase is responsible for Fusarium toxin-degrading activity in a soil Sphingomonas strain".SCIENTIFIC REPORTS 7.-(2017):9549. |
入库方式： OAI收割
来源：上海营养与健康研究所
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