Autoregulation of RBM10 and cross-regulation of RBM10/RBM5 via alternative splicing-coupled nonsense-mediated decay
文献类型：期刊论文
| 作者 | Sun, Yue2,3,4; Saffen, David3,4; Bao, Yufang4; Song, Fan4; Shen, Xianfeng4; Zhao, Jiawei4; Zuo, Ji4; Wang, Yongbo4; Han, Wenjian5; Wang, Zefeng5 |
| 刊名 | NUCLEIC ACIDS RESEARCH
 |
| 出版日期 | 2017 |
| 卷号 | 45期号:14页码:8524-8540 |
| 关键词 | conjugated linoleic acid nitro-fatty acid nitroalkene RNA-Seq vascular smooth muscle cell |
| ISSN号 | 0305-1048 |
| DOI | 10.1093/nar/gkx508 |
| 文献子类 | Article |
| 英文摘要 | Mutations in the spliceosomal RNA binding protein RBM10 cause TARP syndrome and are frequently observed in lung adenocarcinoma (LUAD). We have previously shown that RBM10 enhances exon skipping of its target genes, including its paralog RBM5. Here, we report that RBM10 negatively regulates its own mRNA and protein expression and that of RBM5 by promoting alternative splicing-coupled nonsense-mediated mRNA decay (AS-NMD). Through computational analysis and experimental validation, we identified RBM10-promoted skipping of exon 6 or 12 in RBM10 and exon 6 or 16 in RBM5 as the underlying AS-NMD events. Importantly, we showed that LUAD-associated mutations affecting splice sites of RBM10 exons 6 or 12 abolished exon inclusion and correlated with reduced expression of RBM10 RNA. Together, our investigations have revealed novel molecular mechanisms underlying RBM10 autoregulation and cross-regulation of RBM5, thereby providing in-sights concerning the functions of RBM10 under various physiological and pathological conditions. Our combined computational and experimental approach should be useful for elucidating the role of AS-NMD in auto-and cross-regulation by other splicing regulators. |
| 学科主题 | Biochemistry & Molecular Biology |
| WOS关键词 | MESSENGER-RNA DECAY ; GENOME-WIDE ANALYSIS ; BINDING-PROTEIN ; GENE ; TRANSCRIPTOME ; MECHANISMS ; EXPRESSION ; LANDSCAPE ; ELEMENTS ; REVEALS |
| 语种 | 英语 |
| WOS记录号 | WOS:000407916900043 |
| 出版者 | OXFORD UNIV PRESS |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/901]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所 |
| 作者单位 | 1.Southern Univ Sci & Technol, Dept Biol, Shenzhen 518055, Peoples R China; 2.Fudan Univ, Sch Life Sci, Shanghai 200438, Peoples R China; 3.Fudan Univ, Inst Brain Sci, Shanghai 200032, Peoples R China; 4.Fudan Univ, Sch Basic Med Sci, Dept Cellular & Genet Med, Shanghai 200032, Peoples R China; 5.Chinese Acad Sci, CAS MPG Partner Inst Computat Biol, Key Lab Computat Biol, Shanghai 200031, Peoples R China; 6.Fudan Univ, State Key Lab Med Neurobiol, Shanghai 200032, Peoples R China; 7.Zuse Inst Berlin, Takustr 7, D-14195 Berlin, Germany, |
| 推荐引用方式 GB/T 7714 | Sun, Yue,Saffen, David,Bao, Yufang,et al. Autoregulation of RBM10 and cross-regulation of RBM10/RBM5 via alternative splicing-coupled nonsense-mediated decay[J]. NUCLEIC ACIDS RESEARCH,2017,45(14):8524-8540. |
| APA | Sun, Yue.,Saffen, David.,Bao, Yufang.,Song, Fan.,Shen, Xianfeng.,...&,.(2017).Autoregulation of RBM10 and cross-regulation of RBM10/RBM5 via alternative splicing-coupled nonsense-mediated decay.NUCLEIC ACIDS RESEARCH,45(14),8524-8540. |
| MLA | Sun, Yue,et al."Autoregulation of RBM10 and cross-regulation of RBM10/RBM5 via alternative splicing-coupled nonsense-mediated decay".NUCLEIC ACIDS RESEARCH 45.14(2017):8524-8540. |
入库方式： OAI收割
来源：上海营养与健康研究所
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