Competitive profiling of celastrol targets in human cervical cancer HeLa cells via quantitative chemical proteomics
文献类型：期刊论文
| 作者 | Zhou, Yiqing4; Li, Weichao4; Wang, Mingli4; Xiao, Youli3,4; Li, Weichao3; Zhang, Xixi3; Wang, Mingli2; Tong, Xiaofeng2; Zhang, Xixi1; Zhang, Haibing1 |
| 刊名 | MOLECULAR BIOSYSTEMS
 |
| 出版日期 | 2017 |
| 卷号 | 13期号:1页码:83-91 |
| 关键词 | developmental biology disease model regeneration zebrafish |
| ISSN号 | 1742-206X |
| DOI | 10.1039/c6mb00691d |
| 文献子类 | Article |
| 英文摘要 | Celastrol, isolated from the traditional Chinese medicinal herb Tripterygium wilfordii Hook. f. (Thunder God's Vine), has been used to treat cancer, chronic inflammatory, autoimmune and other human diseases. However, to date, the protein targets and the mechanism of action of celastrol have remained elusive. In this study, we find that celastrol can react with protein thiols in a unique covalent and reversible manner, while protein denaturing disrupts the interaction. Through a competitive chemoproteomics approach utilizing a cysteine-targeting activity-based probe, we report the proteome-wide quantitative profiling of cellular targets of celastrol in human cervical cancer HeLa cells. Representative targets are further validated via in vitro binding experiments and/or enzymatic activity assays. Bioinformatics analysis results suggest that celastrol exerts its numerous therapeutic effects through interaction with promiscuous proteins involved in various biological processes and cellular pathways. |
| 学科主题 | Biochemistry & Molecular Biology |
| WOS关键词 | NF-KAPPA-B ; SMALL MOLECULES ; HEAT-SHOCK ; PROTEIN TARGETS ; IDENTIFICATION ; REACTIVITY ; TRANSFERASES ; ACTIVATION ; PROTEASOME ; CYSTEINES |
| 语种 | 英语 |
| WOS记录号 | WOS:000391697500007 |
| 出版者 | ROYAL SOC CHEMISTRY |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/916]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所 |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Nutr Sci, Shanghai 200032, Peoples R China, 2.East China Univ Sci & Technol, Dept Chem, Shanghai 200001, Peoples R China; 3.Univ Chinese Acad Sci, Beijing 100039, Peoples R China; 4.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, CAS Ctr Excellence Mol Plant Sci,CAS Key Lab Synt, Shanghai 200032, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Zhou, Yiqing,Li, Weichao,Wang, Mingli,et al. Competitive profiling of celastrol targets in human cervical cancer HeLa cells via quantitative chemical proteomics[J]. MOLECULAR BIOSYSTEMS,2017,13(1):83-91. |
| APA | Zhou, Yiqing.,Li, Weichao.,Wang, Mingli.,Xiao, Youli.,Li, Weichao.,...&,.(2017).Competitive profiling of celastrol targets in human cervical cancer HeLa cells via quantitative chemical proteomics.MOLECULAR BIOSYSTEMS,13(1),83-91. |
| MLA | Zhou, Yiqing,et al."Competitive profiling of celastrol targets in human cervical cancer HeLa cells via quantitative chemical proteomics".MOLECULAR BIOSYSTEMS 13.1(2017):83-91. |
入库方式： OAI收割
来源：上海营养与健康研究所
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