Correcting for cell-type heterogeneity in epigenome-wide association studies: revisiting previous analyses
文献类型：期刊论文
| 作者 | Zheng, Shijie C.12,13; Teschendorff, Andrew E.13; Beck, Stephan11; Jaffe, Andrew E.2,10; Koestler, Devin C.1; Hansen, Kasper D.7,8,9; Houseman, Andres E.6; Irizarry, Rafael A.4,5; Teschendorff, Andrew E.3; , |
| 刊名 | NATURE METHODS
 |
| 出版日期 | 2017 |
| 卷号 | 14期号:3页码:216-217 |
| ISSN号 | 1548-7091 |
| DOI | 10.1038/nmeth.4187 |
| 文献子类 | Letter |
| 英文摘要 | Calorie restriction (CR) extends lifespan and elicits numerous effects beneficial to health and metabolism in various model organisms, but the underlying mechanisms are not completely understood. Gut microbiota has been reported to be associated with the beneficial effects of CR; however, it is unknown whether these effects of CR are causally mediated by gut microbiota. In this study, we employed an antibiotic-induced microbiota-depleted mouse model to investigate the functional role of gut microbiota in CR. Depletion of gut microbiota rendered mice resistant to CR-induced loss of body weight, accompanied by the increase in fat mass, the reduction in lean mass and the decline in metabolic rate. Depletion of gut microbiota led to increases in fasting blood glucose and cholesterol levels independent of CR. A few metabolism-modulating hormones including leptin and insulin were altered by CR and/or gut microbiota depletion. In addition, CR altered the composition of gut microbiota with significant increases in major probiotic genera such as Lactobacillus and Bifidobacterium, together with the decrease of Helicobacter. In addition, we performed fecal microbiota transplantation in mice fed with high-fat diet. Mice with transferred microbiota from calorie-restricted mice resisted high fat diet-induced obesity and exhibited metabolic improvement such as alleviated hepatic lipid accumulation. Collectively, these data indicate that CR-induced metabolic improvement especially in body weight reduction is mediated by intestinal microbiota to a certain extent. |
| 学科主题 | Biochemistry & Molecular Biology |
| WOS关键词 | DNA METHYLATION |
| 语种 | 英语 |
| WOS记录号 | WOS:000395661700005 |
| 出版者 | NATURE PUBLISHING GROUP |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/929]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所 |
| 作者单位 | 1.Univ Kansas, Med Ctr, Dept Biostat, Kansas City, KS 66103 USA; 2.Johns Hopkins Bloomberg Sch Publ Hlth, Dept Biostat, Baltimore, MD USA; 3.UCL, Dept Womens Canc, London, England, 4.UCL, UCL Canc Inst, Stat Canc Genom, Paul OGorman Bldg, London, England; 5.Harvard TH Chan Sch Publ Hlth, Dept Biostat, Boston, MA USA; 6.Dana Farber Canc Inst, Dept Biostat & Computat Biol, Boston, MA 02115 USA; 7.Oregon State Univ, Coll Publ Hlth & Human Sci, Sch Biol & Populat Hlth Sci, Corvallis, OR 97331 USA; 8.Johns Hopkins Univ, Sch Med, McKusick Nathans Inst Genet Med, Baltimore, MD USA; 9.Johns Hopkins Univ, Sch Med, Ctr Epigenet, Baltimore, MD USA; 10.Johns Hopkins Univ, Ctr Computat Biol, Baltimore, MD USA; |
| 推荐引用方式 GB/T 7714 | Zheng, Shijie C.,Teschendorff, Andrew E.,Beck, Stephan,et al. Correcting for cell-type heterogeneity in epigenome-wide association studies: revisiting previous analyses[J]. NATURE METHODS,2017,14(3):216-217. |
| APA | Zheng, Shijie C..,Teschendorff, Andrew E..,Beck, Stephan.,Jaffe, Andrew E..,Koestler, Devin C..,...&,.(2017).Correcting for cell-type heterogeneity in epigenome-wide association studies: revisiting previous analyses.NATURE METHODS,14(3),216-217. |
| MLA | Zheng, Shijie C.,et al."Correcting for cell-type heterogeneity in epigenome-wide association studies: revisiting previous analyses".NATURE METHODS 14.3(2017):216-217. |
入库方式： OAI收割
来源：上海营养与健康研究所
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