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CRISPR/Cas9 with single guide RNA expression driven by small tRNA promoters showed reduced editing efficiency compared to a U6 promoter

文献类型：期刊论文

作者Wei, Yuda1,3; Qiu, Yan1,3; Chen, Yanhao1,3; Liu, Gaigai1,3; Zhang, Yongxian1,3; Ding, Qiurong1,3; Xu, Luwei2; ,
刊名RNA
出版日期2017
卷号23期号:1页码:1-5
关键词CRISPR/Cas9 genome editing tRNA promoter
ISSN号1355-8382
DOI10.1261/rna.057596.116
文献子类Article
英文摘要Multiplex genome engineering in vivo with CRISPR/Cas9 shows great promise as a potential therapeutic approach. The ability to incorporate multiple single guide RNA (sgRNA) cassettes together with Cas9 gene expression in one AAV vector could greatly enhance the efficiency. In a recent Method article, Mefferd and coworkers indicated that small tRNA promoters could be used to drive sgRNA expression to facilitate the construction of a more effective AAV vector. In contrast, we found that when targeting endogenous genomic loci, CRISPR/Cas9 with tRNA promoter-driven sgRNA expression showed much reduced genome editing activity, compared with significant cleavage with U6 promoter-driven sgRNA expression. Though the underlying mechanisms are still under investigation, our study suggests that the CRISPR/Cas9 system with tRNA promoterdriven sgRNA expression needs to be reevaluated before it can be used for therapeutic genome editing.
学科主题Biochemistry & Molecular Biology
WOS关键词MUSCULAR-DYSTROPHY ; MOUSE MODEL ; GENOME ; MUSCLE
语种英语
WOS记录号WOS:000391687100001
出版者COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
版本出版稿
源URL[http://202.127.25.144/handle/331004/930]  
专题中国科学院上海生命科学研究院营养科学研究所
作者单位1.Univ Chinese Acad Sci, Shanghai 200031, Peoples R China;
2.Taizhou Hosp Tradit Chinese Med, Taizhou 225300, Peoples R China,
3.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Nutr Sci, CAS Key Lab Nutr & Metab, Shanghai 200031, Peoples R China;
推荐引用方式
GB/T 7714		 Wei, Yuda,Qiu, Yan,Chen, Yanhao,et al. CRISPR/Cas9 with single guide RNA expression driven by small tRNA promoters showed reduced editing efficiency compared to a U6 promoter[J]. RNA,2017,23(1):1-5.
APA Wei, Yuda.,Qiu, Yan.,Chen, Yanhao.,Liu, Gaigai.,Zhang, Yongxian.,...&,.(2017).CRISPR/Cas9 with single guide RNA expression driven by small tRNA promoters showed reduced editing efficiency compared to a U6 promoter.RNA,23(1),1-5.
MLA Wei, Yuda,et al."CRISPR/Cas9 with single guide RNA expression driven by small tRNA promoters showed reduced editing efficiency compared to a U6 promoter".RNA 23.1(2017):1-5.

入库方式： OAI收割

来源：上海营养与健康研究所

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