Development and Validation of a Liquid Chromatography-Tandem Mass Spectrometry Method Coupled with Dispersive Solid-Phase Extraction for Simultaneous Quantification of Eight Paralytic Shellfish Poisoning Toxins in Shellfish
文献类型：期刊论文
| 作者 | Yang, Xianli3; Zhao, Zhiyong3; Nie, Dongxia3; Zhou, Changyan3; Zhou, Lei1; Shi, Xizhi1; Tan, Yanglan4; Liu, Hong2; , |
| 刊名 | TOXINS
 |
| 出版日期 | 2017 |
| 卷号 | 9期号:7页码:206 |
| ISSN号 | 2072-6651 |
| 关键词 | paralytic shellfish poisoning (PSP) toxins LC-MS/MS dispersive solid-phase extraction (dSPE) saxitoxin |
| DOI | 10.3390/toxins9070206 |
| 文献子类 | Article |
| 英文摘要 | In this study, a high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/ MS) method was developed for simultaneous determination of eight paralytic shellfish poisoning (PSP) toxins, including saxitoxin (STX), neosaxitoxin (NEO), gonyautoxins (GTX1-4) and the N-sulfo carbamoyl toxins C1 and C2, in sea shellfish. The samples were extracted by acetonitrile/water (80: 20, v/v) with 0.1% formic and purified by dispersive solid-phase extraction (dSPE) with C18 silica and acidic alumina. Qualitative and quantitative detection for the target toxins were conducted under the multiple reaction monitoring (MRM) mode by using the positive electrospray ionization (ESI) mode after chromatographic separation on a TSK-gel Amide-80 HILIC column with water and acetonitrile. Matrix-matched calibration was used to compensate for matrix effects. The established method was further validated by determining the linearity (R-2 >= 0.9900), average recovery (81.52-116.50%), sensitivity (limits of detection (LODs): 0.33-5.52 mu g.kg(-1); limits of quantitation (LOQs): 1.32-11.29 mu g.kg(-1)) and precision (relative standard deviation (RSD) <= 19.10%). The application of this proposed approach to thirty shellfish samples proved its desirable performance and sufficient capability for simultaneous determination of multiclass PSP toxins in sea foods. |
| 学科主题 | Food Science & Technology ; Toxicology |
| WOS关键词 | HIGH-THROUGHPUT ANALYSIS ; PRECHROMATOGRAPHIC OXIDATION ; QUANTITATIVE-DETERMINATION ; FLUORESCENCE DETECTION ; PSP TOXINS ; SAXITOXIN ; OPTIMIZATION ; MUSSELS ; COLUMN ; MS/MS |
| 语种 | 英语 |
| 出版者 | MDPI AG |
| WOS记录号 | WOS:000407323700004 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/934]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所 |
| 作者单位 | 1.Ningbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R China; 2.Shanghai Municipal Ctr Dis Control & Prevent, Shanghai 200336, Peoples R China, 3.Shanghai Acad Agr Sci, Inst Agrofood Stand & Testing Technol, Lab Qual & Safety risk Assessment Agroprod Shangh, Minist Agr, Shanghai 201403, Peoples R China; 4.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Nutr Sci, Key Lab food safety Res, Shanghai 200031, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Yang, Xianli,Zhao, Zhiyong,Nie, Dongxia,et al. Development and Validation of a Liquid Chromatography-Tandem Mass Spectrometry Method Coupled with Dispersive Solid-Phase Extraction for Simultaneous Quantification of Eight Paralytic Shellfish Poisoning Toxins in Shellfish[J]. TOXINS,2017,9(7):206. |
| APA | Yang, Xianli.,Zhao, Zhiyong.,Nie, Dongxia.,Zhou, Changyan.,Zhou, Lei.,...&,.(2017).Development and Validation of a Liquid Chromatography-Tandem Mass Spectrometry Method Coupled with Dispersive Solid-Phase Extraction for Simultaneous Quantification of Eight Paralytic Shellfish Poisoning Toxins in Shellfish.TOXINS,9(7),206. |
| MLA | Yang, Xianli,et al."Development and Validation of a Liquid Chromatography-Tandem Mass Spectrometry Method Coupled with Dispersive Solid-Phase Extraction for Simultaneous Quantification of Eight Paralytic Shellfish Poisoning Toxins in Shellfish".TOXINS 9.7(2017):206. |
入库方式： OAI收割
来源：上海营养与健康研究所
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