Irisin plays a pivotal role to protect the heart against ischemia and reperfusion injury
文献类型：期刊论文
| 作者 | Wang, Hao6; Zhao, Yu Tina6; Du, Jianfeng6; Zhao, Ting C.6; Zhang, Shouyan2; Dubielecka, Patrycja M.3; Zhuang, Shougang3; Yano, Naohiro5; Chin, Y. Eugene1; Qin, Gangjian4 |
| 刊名 | JOURNAL OF CELLULAR PHYSIOLOGY
 |
| 出版日期 | 2017 |
| 卷号 | 232期号:12页码:3775-3785 |
| 关键词 | cardioprotection heart irisin ischemia reperfusion |
| ISSN号 | 0021-9541 |
| DOI | 10.1002/jcp.25857 |
| 文献子类 | Article |
| 英文摘要 | Irisin, a newly identified hormone, is critical to modulating body metabolism, thermogenesis and reducing oxidative stresses. However, whether irisin protects the heart against myocardial ischemia and reperfusion (I/R) injury remains unknown. In this study, we determine the effect of irisin on myocardial I/R injury in the Langendorff perfused heart and cultured myocytes. Adult C57/BL6 mice were treated with irisin (100mg/kg) or vehicle for 30min to elicit preconditioning. The isolated hearts were subjected to 30min ischemia followed by 30min reperfusion. Left ventricular function was measured and infarction size were determined using by tetrazolium staining. Western blot was employed to determine myocardial SOD-1, active-caspase 3, annexin V, p38, and phospho-p38. H9c2 cardiomyoblasts were exposed to hypoxia and reoxygenation for assessment of the effects of irisin on mitochondrial respiration and mitochondrial permeability transition pore (mPTP). Irisin treatment produced remarkable improvements in ventricular functional recovery, as evident by the increase in RPP and attenuation in LVEDP. As compared to the vehicle treatment, irisin resulted in a marked reduction of myocardial infarct size. Notably, irisin treatment increased SOD-1 and p38 phosphorylation, but suppressed levels of active-caspase 3, cleaved PARP, and annexin V. In cardiomyoblasts exposed to hypoxia/reoxygenation, irisin treatment significantly attenuated hypoxia/reoxygenation (H/R), as indicated by the reduction of lactate dehydrogenase (LDH) leakage and apoptotic cardiomyocytes. Furthermore, irisin treatments suppressed the opening of mPTP, mitochondrial swelling, and protected mitochondria function. Our results indicate that irisin serves as a novel approach to eliciting cardioprotection, which is associated with the improvement of mitochondrial function. |
| 学科主题 | Cell Biology ; Physiology |
| WOS关键词 | MITOCHONDRIAL PERMEABILITY TRANSITION ; PRESERVES MYOCARDIAL PERFORMANCE ; K-ATP CHANNELS ; P38 MAP KINASE ; WHITE FAT ; KAPPA-B ; INHIBITION ; CELLS ; CARDIOPROTECTION ; APOPTOSIS |
| 语种 | 英语 |
| WOS记录号 | WOS:000408155100051 |
| 出版者 | WILEY |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/954]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所 |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Hlth Sci, Key Lab Stem Cell Biol, Shanghai, Peoples R China; 2.Zhengzhou Univ, Luoyang Cent Hosp, Dept Cardiol, Luoyang, Henan, Peoples R China; 3.Brown Univ, Dept Med, Alpert Med Sch, Providence, RI 02912 USA; 4.Northwestern Univ, Feinberg Sch Med, Feinberg Cardiovasc Res Inst, Chicago, IL 60611 USA, 5.Brown Univ, Women & Infants Hosp, Providence, RI USA; 6.Boston Univ, Sch Med, Roger Williams Med Ctr, Dept Surg, 50 Maude St, Providence, RI 02908 USA; |
| 推荐引用方式 GB/T 7714 | Wang, Hao,Zhao, Yu Tina,Du, Jianfeng,et al. Irisin plays a pivotal role to protect the heart against ischemia and reperfusion injury[J]. JOURNAL OF CELLULAR PHYSIOLOGY,2017,232(12):3775-3785. |
| APA | Wang, Hao.,Zhao, Yu Tina.,Du, Jianfeng.,Zhao, Ting C..,Zhang, Shouyan.,...&,.(2017).Irisin plays a pivotal role to protect the heart against ischemia and reperfusion injury.JOURNAL OF CELLULAR PHYSIOLOGY,232(12),3775-3785. |
| MLA | Wang, Hao,et al."Irisin plays a pivotal role to protect the heart against ischemia and reperfusion injury".JOURNAL OF CELLULAR PHYSIOLOGY 232.12(2017):3775-3785. |
入库方式： OAI收割
来源：上海营养与健康研究所
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