A quite sensitive fluorescent loop-mediated isothermal amplification for rapid detection of respiratory syncytial virus
文献类型：期刊论文
| 作者 | Hu, Yihong2; Zhou, Yi2; Liu, Jia2; Zhang, Chiyu2; Wan, Zhenzhou3; Mu, Yonglin4; Lan, Ke1; , |
| 刊名 | JOURNAL OF INFECTION IN DEVELOPING COUNTRIES
 |
| 出版日期 | 2019 |
| 卷号 | 13期号:12页码:1135+ |
| 关键词 | Reverse-transcription loop-mediated isothermal amplification respiratory syncytial virus SYTO 9 sensitivity specificity clinical diagnosis |
| ISSN号 | 1972-2680 |
| DOI | 10.3855/jidc.11549 |
| 文献子类 | Article |
| 英文摘要 | Introduction: Human respiratory syncytial virus (hRSV) is a common respiratory virus closely related to respiratory tract infection (RTI). Rapid and accurate detection of hRSV is urgently needed to reduce the high morbidity and mortality due to hRSV infection. Methodology: Here, we established a highly sensitive and specific reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay for the rapid detection of A and B group hRSV simultaneously. The specific primer sets for hRSV A and B groups were designed in the M and M2-2 gene, respectively. SYTO 9 was used as the fluorescent dye for real-time monitoring of the amplification of hRSV RNA without cross reaction between hRSV A and B. Results: The limit of detection (LOD) of our new method was 281.17 50% tissue culture infective doses (TCID50)/mL for hRSV A and 1.58 TCID50/mL for hRSV B. Using 90 clinical samples, a comparison to traditional RT-PCR was performed to validate this assay. The positivity rate of RT-LAMP and RT-PCR were 67.8% and 55.6%, respectively, and the positivity rate of RT-LAMP was significantly higher than RTPCR (chi(2) test, P < 0.01). Conclusions: Compared with traditional RT-PCR method, the newly developed fluorescent RT-LAMP combined with well-designed primers and SYTO 9 is quite sensitive, specific, rapid and well applicable to hRSV clinical diagnosis. |
| 学科主题 | Science & Technology - Other Topics |
| WOS关键词 | VISUAL DETECTION ; INFECTION ; PCR ; RSV ; DIAGNOSIS ; CHILDREN ; LAMP ; REINFECTION ; SHANGHAI ; INFANTS |
| 语种 | 英语 |
| CSCD记录号 | CSCD:32088701 |
| WOS记录号 | WOS:000506637900008 |
| 出版者 | J INFECTION DEVELOPING COUNTRIES |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/958]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所 |
| 作者单位 | 1.Wuhan Univ, Coll Life Sci, Med Res Inst, State Key Lab Virol, Wuhan 430072, Peoples R China, 2.Chinese Acad Sci, Inst Pasteur Shanghai, Pathogen Discovery & Big Data Ctr, Shanghai 200031, Peoples R China; 3.Taizhou Fourth Peoples Hosp, Med Lab, Taizhou, Peoples R China; 4.Chinese Acad Sci, Max Planck Partner Inst Computat Biol, Key Lab Computat Biol, Shanghai, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Hu, Yihong,Zhou, Yi,Liu, Jia,et al. A quite sensitive fluorescent loop-mediated isothermal amplification for rapid detection of respiratory syncytial virus[J]. JOURNAL OF INFECTION IN DEVELOPING COUNTRIES,2019,13(12):1135+. |
| APA | Hu, Yihong.,Zhou, Yi.,Liu, Jia.,Zhang, Chiyu.,Wan, Zhenzhou.,...&,.(2019).A quite sensitive fluorescent loop-mediated isothermal amplification for rapid detection of respiratory syncytial virus.JOURNAL OF INFECTION IN DEVELOPING COUNTRIES,13(12),1135+. |
| MLA | Hu, Yihong,et al."A quite sensitive fluorescent loop-mediated isothermal amplification for rapid detection of respiratory syncytial virus".JOURNAL OF INFECTION IN DEVELOPING COUNTRIES 13.12(2019):1135+. |
入库方式： OAI收割
来源：上海营养与健康研究所
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