Chromatin-sensitive cryptic promoters putatively drive expression of alternative protein isoforms in yeast
文献类型：期刊论文
| 作者 | Wei, Wu1,2,9; Steinmetz, Lars M.2,3; Hennig, Bianca P.3; Chabbert, Christophe D.3; Wang, Jingwen4; Zhang, Yujie4; Sanchez, Yerma Pareja4; Pelechano, Vicent4; Piazza, Ilaria5; Adjalley, Sophie H.6 |
| 刊名 | GENOME RESEARCH
 |
| 出版日期 | 2019 |
| 卷号 | 29期号:12页码:1974-1984 |
| 关键词 | Ca2+ signaling Ca2+ release Ca2+ extrusion Ca2+ channels Ca2+ pumps human embryonic stem cells |
| ISSN号 | 1088-9051 |
| DOI | 10.1101/gr.243378.118 |
| 文献子类 | Article |
| 英文摘要 | Cryptic transcription is widespread and generates a heterogeneous group of RNA molecules of unknown function. To improve our understanding of cryptic transcription, we investigated their transcription start site (TSS) usage, chromatin organization, and posttranscriptional consequences in Saccharomyces cerevisiae. We show that TSSs of chromatin-sensitive internal cryptic transcripts retain comparable features of canonical TSSs in terms of DNA sequence, directionality, and chromatin accessibility. We define the 5' and 3' boundaries of cryptic transcripts and show that, contrary to RNA degradation-sensitive ones, they often overlap with the end of the gene, thereby using the canonical polyadenylation site, and associate to polyribosomes. We show that chromatin-sensitive cryptic transcripts can be recognized by ribosomes and may produce truncated polypeptides from downstream, in-frame start codons. Finally, we confirm the presence of the predicted polypeptides by reanalyzing N-terminal proteomic data sets. Our work suggests that a fraction of chromatin-sensitive internal cryptic promoters initiates the transcription of alternative truncated mRNA isoforms. The expression of these chromatin-sensitive isoforms is conserved from yeast to human, expanding the functional consequences of cryptic transcription and proteome complexity. |
| 学科主题 | Chemistry ; Pharmacology & Pharmacy |
| WOS关键词 | TRANSCRIPTION START SITES ; MESSENGER-RNA ; INTERGENIC TRANSCRIPTION ; SACCHAROMYCES-CEREVISIAE ; BIDIRECTIONAL PROMOTERS ; ANTISENSE TRANSCRIPTION ; TRANSLATION ; QUANTIFICATION ; ELONGATION ; REPRESS |
| 语种 | 英语 |
| CSCD记录号 | CSCD:31740578 |
| WOS记录号 | WOS:000500554200005 |
| 出版者 | COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/966]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所 |
| 作者单位 | 1.Shanghai Jiao Tong Univ, Shanghai Childrens Hosp, Shanghai Engn Res Ctr Big Data Pediat Precis Med, Ctr Biomed Informat, Shanghai 200040, Peoples R China; 2.Stanford Univ, Stanford Genome Technol Ctr, Palo Alto, CA 94304 USA; 3.EMBL, Genome Biol Unit, D-69117 Heidelberg, Germany; 4.Karolinska Inst, Dept Microbiol Tumor & Cell Biol, SciLifeLab, S-17165 Solna, Sweden; 5.Swiss Fed Inst Technol, Inst Mol Syst Biol, Dept Biol, CH-8093 Zurich, Switzerland; 6.Wellcome Sanger Inst, Hinxton CB10 1SA, England; 7.Stanford Univ, Sch Med, Dept Genet, Stanford, CA 94305 USA; 8.Roche Innovat Ctr Zurich, CH-8952 Schlieren, Switzerland, 9.Chinese Acad Sci, Univ Chinese Acad Sci, Shanghai Inst Biol Sci,CAS Key Lab Computat Biol, Shanghai Inst Nutr & Hlth,CAS MPG Partner Inst Co, Shanghai 200031, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Wei, Wu,Steinmetz, Lars M.,Hennig, Bianca P.,et al. Chromatin-sensitive cryptic promoters putatively drive expression of alternative protein isoforms in yeast[J]. GENOME RESEARCH,2019,29(12):1974-1984. |
| APA | Wei, Wu.,Steinmetz, Lars M..,Hennig, Bianca P..,Chabbert, Christophe D..,Wang, Jingwen.,...&,.(2019).Chromatin-sensitive cryptic promoters putatively drive expression of alternative protein isoforms in yeast.GENOME RESEARCH,29(12),1974-1984. |
| MLA | Wei, Wu,et al."Chromatin-sensitive cryptic promoters putatively drive expression of alternative protein isoforms in yeast".GENOME RESEARCH 29.12(2019):1974-1984. |
入库方式： OAI收割
来源：上海营养与健康研究所
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