Genetic tracing of hepatocytes in liver homeostasis, injury, and regeneration
文献类型：期刊论文
| 作者 | Wang, Yue1,2; Huang, XiuZhen1,2; He, Lingjuan1,2; Pu, Wenjuan1,2; Li, Yan1,2; Liu, Qiaozhen1,2; Li, Yi1,2; Zhang, Libo1,2; Yu, Wei1,2; Zhao, Huan1,2 |
| 刊名 | JOURNAL OF BIOLOGICAL CHEMISTRY
 |
| 出版日期 | 2017 |
| 卷号 | 292期号:21页码:8594-8604 |
| ISSN号 | 0021-9258 |
| 关键词 | PI3K Peptide Therapy Gastric cancers Mouse model |
| DOI | 10.1074/jbc.M117.782029 |
| 文献子类 | Article |
| 英文摘要 | The liver possesses a remarkable capacity to regenerate after damage. There is a heated debate on the origin of new hepatocytes after injuries in adult liver. Hepatic stem/progenitor cells have been proposed to produce functional hepatocytes after injury. Recent studies have argued against this model and suggested that pre-existing hepatocytes, rather than stem cells, contribute new hepatocytes. This hepatocyte-to-hepatocyte model is mainly based on labeling of hepatocytes with Cre-recombinase delivered by the adeno-associated virus. However, the impact of virus infection on cell fate determination, consistency of infection efficiency, and duration of Cre-virus in hepatocytes remain confounding factors that interfere with the data interpretation. Here, we generated a new genetic tool Alb-DreER to label almost all hepatocytes (>99.5%) and track their contribution to different cell lineages in the liver. By pulse-and-chase strategy, we found that pre-existing hepatocytes labeled by Alb-DreER contribute to almost all hepatocytes during normal homeostasis and after liver injury. Virtually all hepatocytes in the injured liver are descendants of pre-existing hepatocytes through self-expansion. We concluded that stem cell differentiation is unlikely to be responsible for the generation of a substantial number of new hepatocytes in adult liver. Our study also provides a new mouse tool for more precise in vivo genetic study of hepatocytes in the field. |
| 学科主题 | Biochemistry & Molecular Biology |
| WOS关键词 | PROGENITOR CELLS ; MOUSE-LIVER ; PERIPORTAL HEPATOCYTES ; MATURE HEPATOCYTES ; STEM-CELLS ; RISE ; DIFFERENTIATION ; CHOLANGIOCYTES ; CONTRIBUTES ; RATHER |
| 语种 | 英语 |
| 出版者 | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC |
| WOS记录号 | WOS:000402153900008 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/976]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所 |
| 作者单位 | 1.Univ Chinese Acad Sci, CAS Ctr Excellence Mol Cell Sci, Shanghai Inst Biochem & Cell Biol, State Key Lab Cell Biol,Chinese Acad Sci, Shanghai 200031, Peoples R China; 2.Univ Chinese Acad Sci, Chinese Acad Sci, Shanghai Inst Biol Sci, Key Lab Nutr & Metab,Inst Nutr Sci, Shanghai 200031, Peoples R China; 3.Tongji Univ, Sch Med, Shanghai Peoples Hosp 10, Dept Gastroenterol, 301 Yanchang Rd, Shanghai 200072, Peoples R China; 4.Jinan Univ, Inst Aging & Regenerat Med, Minist Educ, Key Lab Regenerat Med, Guangzhou 510632, Guangdong, Peoples R China; 5.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China, |
| 推荐引用方式 GB/T 7714 | Wang, Yue,Huang, XiuZhen,He, Lingjuan,et al. Genetic tracing of hepatocytes in liver homeostasis, injury, and regeneration[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2017,292(21):8594-8604. |
| APA | Wang, Yue.,Huang, XiuZhen.,He, Lingjuan.,Pu, Wenjuan.,Li, Yan.,...&,.(2017).Genetic tracing of hepatocytes in liver homeostasis, injury, and regeneration.JOURNAL OF BIOLOGICAL CHEMISTRY,292(21),8594-8604. |
| MLA | Wang, Yue,et al."Genetic tracing of hepatocytes in liver homeostasis, injury, and regeneration".JOURNAL OF BIOLOGICAL CHEMISTRY 292.21(2017):8594-8604. |
入库方式： OAI收割
来源：上海营养与健康研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。