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Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion

文献类型：期刊论文

作者Wang, Xiao1,4,5; Li, Jianan1,4,5; Wu, Jing4; Wang, Ruixuan4; Huang, Xingxu4; Chen, Jia4; Wang, Ying2; Wei, Jia2; Yang, Li2; Yang, Bei3
刊名NATURE BIOTECHNOLOGY
出版日期2018
卷号36期号:10页码:946-+
关键词gene expression pattern Monte-Carlo feature selection method neural stem cell
ISSN号1087-0156
DOI10.1038/nbt.4198
文献子类Article
英文摘要Base editors (BEs) enable the generation of targeted single-nucleotide mutations, but currently used rat APOBEC1-based BEs are relatively inefficient in editing cytosines in highly methylated regions or in GpC contexts. By screening a variety of APOBEC and AID deaminases, we show that human APOBEC3A-conjugated BEs and versions we engineered to have narrower editing windows can mediate efficient C-to-T base editing in regions with high methylation levels and GpC dinucleotide content.
学科主题Biotechnology & Applied Microbiology
WOS关键词CYTOSINE DEAMINATION ; DNA DEMETHYLATION ; STRANDED-DNA ; APOBEC
语种英语
WOS记录号WOS:000447001600013
出版者NATURE PUBLISHING GROUP
版本出版稿
源URL[http://202.127.25.144/handle/331004/984]  
专题中国科学院上海生命科学研究院营养科学研究所
作者单位1.Univ Chinese Acad Sci, Beijing, Peoples R China;
2.Univ Chinese Acad Sci, Chinese Acad Sci, Shanghai Inst Biol Sci,Shanghai Inst Nutr & Hlth, Key Lab Computat Biol,CAS MPG Partner Inst Comput, Shanghai, Peoples R China;
3.ShanghaiTech Univ, Shanghai Inst Adv Immunochem Studies, Shanghai, Peoples R China,
4.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China;
5.Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, Shanghai, Peoples R China;
推荐引用方式
GB/T 7714		 Wang, Xiao,Li, Jianan,Wu, Jing,et al. Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion[J]. NATURE BIOTECHNOLOGY,2018,36(10):946-+.
APA Wang, Xiao.,Li, Jianan.,Wu, Jing.,Wang, Ruixuan.,Huang, Xingxu.,...&,.(2018).Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion.NATURE BIOTECHNOLOGY,36(10),946-+.
MLA Wang, Xiao,et al."Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion".NATURE BIOTECHNOLOGY 36.10(2018):946-+.

入库方式： OAI收割

来源：上海营养与健康研究所

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