CDK9 modulates circadian clock by attenuating REV-ERB alpha activity
文献类型：期刊论文
| 作者 | Ou, Jiali1; Li, Huilin1; Tang, Yun-Chi1; Qiu, Peiyuan2; Li, Qing2; Chang, Hung-Chun2; , |
| 刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
 |
| 出版日期 | 2019 |
| 卷号 | 513期号:4页码:967-973 |
| 关键词 | Circadian clock CDK9 BMAL1 REV-ERBa PER2 Suprachiasmatic nucleus |
| ISSN号 | 0006-291X |
| DOI | 10.1016/j.bbrc.2019.04.043 |
| 文献子类 | Article |
| 英文摘要 | Circadian clock and cell cycle are vital cellular programs acting in a timely-regulated, cyclic manner. The two cellular oscillators are coupled in various ways to facilitate biological processes. Here we report CDK9, a kinase belongs to the CDK family in regulating cell cycle and RNA Pol II activity, can serve as a modulator for circadian clock. We identified CDK inhibitor LY2857785 potently blocked PER2:LUC expression in MEFs from a screen of 17 commonly-used CDK inhibitors. We further analyzed the possible targets of LY2857785 by siRNA approach, and confirmed CDK9 as the main effector. LY2857785 treatment, as well as Cdk9 knock-down, led to lowered expression of Bmall in accordance with elevated expression of Rev-Erb alpha. CDK9 associated with REV-ERBa thus attenuated REV-ERB alpha binding to the RORE for Bmall suppression. To conform the circadian-modulating activity of CDK9 in vivo, we knocked down CDK9 in mice at the anterior hypothalamus covering the central oscillator SCN, and found the respiratory exchange ratio, daily activity and circadian period were altered in the Cdk9-knockdown mice. Together, our finding designated CDK9 as a novel modulator in circadian clock. CDK9 may serve as a vital basis to understand circadian- and cell cycle-misregulated ailments such as cancer. (C) 2019 The Authors. Published by Elsevier Inc. |
| 学科主题 | Biochemistry & Molecular Biology ; Biophysics |
| WOS关键词 | CELL-CYCLE ; TRANSCRIPTIONAL ARCHITECTURE ; RESPONSE ELEMENT ; METABOLISM ; CRYPTOCHROME ; EXPRESSION ; INHIBITOR ; BEHAVIOR ; CANCER |
| 语种 | 英语 |
| WOS记录号 | WOS:000468856400031 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/988]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所 |
| 作者单位 | 1.Shanghai Jiao Tong Univ, Univ Chinese Acad Sci,Chinese Acad Sci, CAS Key Lab Tissue Microenvironm & Tumor,Sch Med, Shanghai Inst Nutr & Hlth,Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China; 2.Chinese Acad Sci, CAS Ctr Excellence Brain Sci & Intelligence Techn, CAS Key Lab Primate Neurobiol, Inst Neurosci, Shanghai 200031, Peoples R China, |
| 推荐引用方式 GB/T 7714 | Ou, Jiali,Li, Huilin,Tang, Yun-Chi,et al. CDK9 modulates circadian clock by attenuating REV-ERB alpha activity[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2019,513(4):967-973. |
| APA | Ou, Jiali.,Li, Huilin.,Tang, Yun-Chi.,Qiu, Peiyuan.,Li, Qing.,...&,.(2019).CDK9 modulates circadian clock by attenuating REV-ERB alpha activity.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,513(4),967-973. |
| MLA | Ou, Jiali,et al."CDK9 modulates circadian clock by attenuating REV-ERB alpha activity".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 513.4(2019):967-973. |
入库方式： OAI收割
来源：上海营养与健康研究所
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