A Multispecific Investigation of the Metal Effect in Mammalian Odorant Receptors for Sulfur-Containing Compounds
文献类型：期刊论文
| 作者 | Zhang, Ruina1; Pan, Yi1; Zhang, Yuetian1; Zhuang, Hanyi1; Ahmed, Lucky2; Batista, Victor S.2; Block, Eric3; Zhuang, Hanyi4; , |
| 刊名 | CHEMICAL SENSES
 |
| 出版日期 | 2018 |
| 卷号 | 43期号:5页码:357-366 |
| 关键词 | ancestral reconstruction copper odorant receptors orthologs sulfur |
| ISSN号 | 0379-864X |
| DOI | 10.1093/chemse/bjy022 |
| 文献子类 | Article |
| 英文摘要 | Metal-coordinating compounds are generally known to have strong smells, a phenomenon that can be attributed to the fact that odorant receptors for intense-smelling compounds, such as those containing sulfur, may be metalloproteins. We previously identified a mouse odorant receptor (OR), Olfr1509, that requires copper ions for sensitive detection of a series of metal-coordinating odorants, including (methylthio)methanethiol (MTMT), a strong-smelling component of male mouse urine that attracts female mice. By combining mutagenesis and quantum mechanics/ molecular mechanics (QM/MM) modeling, we identified candidate binding sites in Olfr1509 that may bind to the copper-MTMT complex. However, whether there are other receptors utilizing metal ions for ligand-binding and other sites important for receptor activation is still unknown. In this study, we describe a second mouse OR for MTMT with a copper effect, namely Olfr1019. In an attempt to investigate the functional changes of metal-coordinating ORs in multiple species and to decipher additional sites involved in the metal effect, we cloned various mammalian orthologs of the 2 mouse MTMT receptors, and a third mouse MTMT receptor, Olfr15, that does not have a copper effect. We found that the function of all 3 MTMT receptors varies greatly among species and that the response to MTMT always co-occurred with the copper effect. Furthermore, using ancestral reconstruction and QM/MM modeling combined with receptor functional assay, we found that the amino acid residue R260 in Olfr1509 and the respective R261 site in Olfr1019 may be important for receptor activation. |
| 学科主题 | Behavioral Sciences ; Food Science & Technology ; Neurosciences & Neurology ; Physiology |
| WOS关键词 | OLFACTORY SENSORY NEURONS ; MOUSE SEPTAL ORGAN ; ZINC NANOPARTICLES ; PHYLOGENETIC ANALYSIS ; TRANSITION-ELEMENTS ; MAXIMUM-LIKELIHOOD ; RESPONSE PROFILE ; BINDING-SITE ; EVOLUTION ; BROAD |
| 语种 | 英语 |
| WOS记录号 | WOS:000433236600028 |
| 出版者 | OXFORD UNIV PRESS |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/1014]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所 |
| 作者单位 | 1.Shanghai Jiao Tong Univ, Dept Pathophysiol, Key Lab Cell Differentiat & Apoptosis, Chinese Minist Educ,Sch Med, 227 South Chongqing Rd, Shanghai 200025, Peoples R China; 2.Yale Univ, Dept Chem, 225 Prospect St, New Haven, CT 06520 USA; 3.SUNY Albany, Dept Chem, 1400 Washington Ave, Albany, NY 12222 USA; 4.Shanghai Jiao Tong Univ, Sch Med, Inst Hlth Sci, Shanghai Inst Biol Sci,Chinese Acad Sci, 320 Yueyang Rd, Shanghai 200031, Peoples R China, |
| 推荐引用方式 GB/T 7714 | Zhang, Ruina,Pan, Yi,Zhang, Yuetian,et al. A Multispecific Investigation of the Metal Effect in Mammalian Odorant Receptors for Sulfur-Containing Compounds[J]. CHEMICAL SENSES,2018,43(5):357-366. |
| APA | Zhang, Ruina.,Pan, Yi.,Zhang, Yuetian.,Zhuang, Hanyi.,Ahmed, Lucky.,...&,.(2018).A Multispecific Investigation of the Metal Effect in Mammalian Odorant Receptors for Sulfur-Containing Compounds.CHEMICAL SENSES,43(5),357-366. |
| MLA | Zhang, Ruina,et al."A Multispecific Investigation of the Metal Effect in Mammalian Odorant Receptors for Sulfur-Containing Compounds".CHEMICAL SENSES 43.5(2018):357-366. |
入库方式： OAI收割
来源：上海营养与健康研究所
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