Single-Cell Transcriptomic Analysis of Cardiac Differentiation from Human PSCs Reveals HOPX-Dependent Cardiomyocyte Maturation
文献类型：期刊论文
| 作者 | Friedman, Clayton E.8,9; Quan Nguyen8; Lukowski, Samuel W.8; Helfer, Abbigail8,9; Chiu, Han Sheng8,9; Baillie, Greg J.8; Senabouth, Anne8; Christ, Angelika N.8; Bruxner, Timothy J.8; Powell, Joseph E.8 |
| 刊名 | CELL STEM CELL
 |
| 出版日期 | 2018 |
| 卷号 | 23期号:4页码:586-+ |
| 关键词 | Graves' disease Thyroid hormone Atrial fibrillation MicroRNA |
| ISSN号 | 1934-5909 |
| DOI | 10.1016/j.stem.2018.09.009 |
| 文献子类 | Article |
| 英文摘要 | Cardiac differentiation of human pluripotent stem cells (hPSCs) requires orchestration of dynamic gene regulatory networks during stepwise fate transitions but often generates immature cell types that do not fully recapitulate properties of their adult counterparts, suggesting incomplete activation of key transcriptional networks. We performed extensive single-cell transcriptomic analyses to map fate choices and gene expression programs during cardiac differentiation of hPSCs and identified strategies to improve in vitro cardiomyocyte differentiation. Utilizing genetic gain- and loss-of-function approaches, we found that hypertrophic signaling is not effectively activated during monolayer-based cardiac differentiation, thereby preventing expression of HOPX and its activation of downstream genes that govern late stages of cardiomyocyte maturation. This study therefore provides a key transcriptional roadmap of in vitro cardiac differentiation at single-cell resolution, revealing fundamental mechanisms underlying heart development and differentiation of hPSC-derived cardiomyocytes. |
| 学科主题 | Cell Biology |
| WOS关键词 | PLURIPOTENT STEM-CELLS ; GENE-EXPRESSION ; HOMEODOMAIN PROTEIN ; RNA-SEQ ; HYPERTROPHY ; MODULATION ; MOUSE |
| 语种 | 英语 |
| WOS记录号 | WOS:000446342800017 |
| 出版者 | CELL PRESS |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/1042]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所 |
| 作者单位 | 1.Univ Washington, Inst Stem Cell & Regenerat Med, Dept Pathol, Seattle, WA 98195 USA; 2.Univ Washington, Inst Stem Cell & Regenerat Med, Dept Biochem, Seattle, WA 98195 USA; 3.Univ Washington, Inst Stem Cell & Regenerat Med, Dept Bioengn & Med Cardiol, Seattle, WA 98195 USA; 4.Carnegie Mellon Univ, Sch Comp Sci, Computat Biol Dept, Pittsburgh, PA 15213 USA; 5.Univ Washington, Paul G Allen Sch Comp Sci & Engn, Seattle, WA 98195 USA; 6.Univ Sydney, Sydney Med Sch, Sch Med Sci, Sydney, NSW 2006, Australia; 7.Queensland Inst Med Res, Brisbane, Qld 4006, Australia, 8.Univ Queensland, Inst Mol Biosci, Brisbane, Qld 4072, Australia; 9.Univ Queensland, Ctr Cardiac & Vasc Biol, Brisbane, Qld 4072, Australia; 10.Univ Queensland, Sch Biomed Sci, Brisbane, Qld 4072, Australia; |
| 推荐引用方式 GB/T 7714 | Friedman, Clayton E.,Quan Nguyen,Lukowski, Samuel W.,et al. Single-Cell Transcriptomic Analysis of Cardiac Differentiation from Human PSCs Reveals HOPX-Dependent Cardiomyocyte Maturation[J]. CELL STEM CELL,2018,23(4):586-+. |
| APA | Friedman, Clayton E..,Quan Nguyen.,Lukowski, Samuel W..,Helfer, Abbigail.,Chiu, Han Sheng.,...&,.(2018).Single-Cell Transcriptomic Analysis of Cardiac Differentiation from Human PSCs Reveals HOPX-Dependent Cardiomyocyte Maturation.CELL STEM CELL,23(4),586-+. |
| MLA | Friedman, Clayton E.,et al."Single-Cell Transcriptomic Analysis of Cardiac Differentiation from Human PSCs Reveals HOPX-Dependent Cardiomyocyte Maturation".CELL STEM CELL 23.4(2018):586-+. |
入库方式： OAI收割
来源：上海营养与健康研究所
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