Genetic Lineage Tracing of Nonmyocyte Population by Dual Recombinases
文献类型：期刊论文
| 作者 | Li, Yan2,3; He, Lingjuan2,3; Huang, Xiuzhen2,3; Zhao, Huan2,3; Zhang, Shaohua2,3; Pu, Wenjuan2,3; Tian, Xueying2,3; Li, Yi2,3; Liu, Qiaozhen2,3; Yu, Wei2,3 |
| 刊名 | CIRCULATION
 |
| 出版日期 | 2018 |
| 卷号 | 138期号:8页码:793-805 |
| 关键词 | cell lineage myocardial infarction myocardial revascularization myocytes cardiac recombinases stem cell |
| ISSN号 | 0009-7322 |
| DOI | 10.1161/CIRCULATIONAHA.118.034250 |
| 文献子类 | Article |
| 英文摘要 | Background: Whether the adult mammalian heart harbors cardiac stem cells for regeneration of cardiomyocytes is an important yet contentious topic in the field of cardiovascular regeneration. The putative myocyte stem cell populations recognized without specific cell markers, such as the cardiosphere-derived cells, or with markers such as Sca1(+), Bmi1(+), Isl1(+), or Abcg2(+) cardiac stem cells have been reported. Moreover, it remains unclear whether putative cardiac stem cells with unknown or unidentified markers exist and give rise to de novo cardiomyocytes in the adult heart. Methods: To address this question without relying on a particular stem cell marker, we developed a new genetic lineage tracing system to label all nonmyocyte populations that contain putative cardiac stem cells. Using dual lineage tracing system, we assessed whether nonmyocytes generated any new myocytes during embryonic development, during adult homeostasis, and after myocardial infarction. Skeletal muscle was also examined after injury for internal control of new myocyte generation from nonmyocytes. Results: By this stem cell marker-free and dual recombinases-mediated cell tracking approach, our fate mapping data show that new myocytes arise from nonmyocytes in the embryonic heart, but not in the adult heart during homeostasis or after myocardial infarction. As positive control, our lineage tracing system detected new myocytes derived from nonmyocytes in the skeletal muscle after injury. Conclusions: This study provides in vivo genetic evidence for nonmyocyte to myocyte conversion in embryonic but not adult heart, arguing again the myogenic potential of putative stem cell populations for cardiac regeneration in the adult stage. This study also provides a new genetic strategy to identify endogenous stem cells, if any, in other organ systems for tissue repair and regeneration. |
| 学科主题 | Cardiovascular System & Cardiology |
| WOS关键词 | CARDIAC STEM-CELLS ; ZEBRAFISH HEART REGENERATION ; SELF-DUPLICATION RATHER ; VASCULAR SMOOTH-MUSCLE ; CRE KNOCK-INS ; C-KIT LOCUS ; ADULT HEART ; ENDOTHELIAL-CELLS ; MAMMALIAN HEART ; C-KIT(+) CELLS |
| 语种 | 英语 |
| WOS记录号 | WOS:000442243600008 |
| 出版者 | LIPPINCOTT WILLIAMS & WILKINS |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/1047]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所 |
| 作者单位 | 1.Univ Munster, Max Planck Inst Mol Biomed, Dept Tissue Morphogenesis, Fac Med, Munster, Germany; 2.Univ Chinese Acad Sci, State Key Lab Cell Biol, CAS Ctr Excellence Mol Cell Sci, Shanghai Inst Biochem & Cell Biol, Beijing, Peoples R China; 3.Univ Chinese Acad Sci, Key Lab Nutr & Metab, Inst Nutr Sci, Shanghai Inst Biol Sci, Beijing, Peoples R China; 4.Kings Coll London, British Heart Fdn Ctr, Cardiovasc Div, London, England; 5.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China; 6.Jinan Univ, Minist Educ, Key Lab Regenerat Med, Jinan, Shandong, Peoples R China; 7.Chinese Univ Hong Kong, Li Ka Shing Inst Hlth Sci, Prince Wales Hosp, Dept Chem Pathol, Shatin, Hong Kong, Peoples R China, |
| 推荐引用方式 GB/T 7714 | Li, Yan,He, Lingjuan,Huang, Xiuzhen,et al. Genetic Lineage Tracing of Nonmyocyte Population by Dual Recombinases[J]. CIRCULATION,2018,138(8):793-805. |
| APA | Li, Yan.,He, Lingjuan.,Huang, Xiuzhen.,Zhao, Huan.,Zhang, Shaohua.,...&,.(2018).Genetic Lineage Tracing of Nonmyocyte Population by Dual Recombinases.CIRCULATION,138(8),793-805. |
| MLA | Li, Yan,et al."Genetic Lineage Tracing of Nonmyocyte Population by Dual Recombinases".CIRCULATION 138.8(2018):793-805. |
入库方式： OAI收割
来源：上海营养与健康研究所
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