Real-time visualization of clustering and intracellular transport of gold nanoparticles by correlative imaging
文献类型：期刊论文
| 作者 | Liu, Mengmeng2,3,4; Li, Qian2,3; Liang, Le2,3; Li, Jiang2,3; Wang, Kun2,3; Li, Jiajun2,3; Lv, Min2,3; Chen, Nan2,3; Wang, Lihua2,3; Fan, Chunhai2,3 |
| 刊名 | NATURE COMMUNICATIONS
 |
| 出版日期 | 2017 |
| 卷号 | 8期号:-页码:15646 |
| ISSN号 | 2041-1723 |
| 关键词 | Tryptophan metabolism Esophageal squamous cell carcinoma Lymph node metastasis Liquid chromatography Tandem mass spectrometry |
| DOI | 10.1038/ncomms15646 |
| 文献子类 | Article |
| 英文摘要 | Mechanistic understanding of the endocytosis and intracellular trafficking of nanoparticles is essential for designing smart theranostic carriers. Physico-chemical properties, including size, clustering and surface chemistry of nanoparticles regulate their cellular uptake and transport. Significantly, even single nanoparticles could cluster intracellularly, yet their clustering state and subsequent trafficking are not well understood. Here, we used DNA-decorated gold (fPlas-gold) nanoparticles as a dually emissive fluorescent and plasmonic probe to examine their clustering states and intracellular transport. Evidence from correlative fluorescence and plasmonic imaging shows that endocytosis of fPlas-gold follows multiple pathways. In the early stages of endocytosis, fPlas-gold nanoparticles appear mostly as single particles and they cluster during the vesicular transport and maturation. The speed of encapsulated fPlas-gold transport was critically dependent on the size of clusters but not on the types of organelle such as endosomes and lysosomes. Our results provide key strategies for engineering theranostic nanocarriers for efficient health management. |
| 学科主题 | Science & Technology - Other Topics |
| WOS关键词 | CLATHRIN-MEDIATED ENDOCYTOSIS ; CELL ; MECHANISMS ; MICROSCOPY ; ENDOSOMES ; DYNAMICS ; PLATFORM ; MOTORS ; CANCER ; VIRUS |
| 语种 | 英语 |
| 出版者 | NATURE PUBLISHING GROUP |
| WOS记录号 | WOS:000402395100001 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/1079]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所 |
| 作者单位 | 1.Univ Oxford, Kellogg Coll, Oxford OX2 6PN, England; 2.Chinese Acad Sci, Div Phys Biol, Shanghai 201800, Peoples R China; 3.Chinese Acad Sci, Bioimaging Ctr, Shanghai Synchrotron Radiat Facil, CAS Key Lab Interfacial Phys & Technol,Shanghai I, Shanghai 201800, Peoples R China; 4.Shanghai East Hosp, Inst Translat Nanomed, Shanghai 200120, Peoples R China; 5.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Nutr Sci, Key Lab food safety Res, Shanghai 200031, Peoples R China; 6.UCB Pharma, Slough SL1 3WE, Berks, England, 7.Univ Calif San Diego, Dept Bioengn Mat Sci & Engn, Inst Engn Med, La Jolla, CA 92093 USA; 8.Univ Calif San Diego, Dept Mech & Aerosp Engn, Inst Engn Med, La Jolla, CA 92093 USA; |
| 推荐引用方式 GB/T 7714 | Liu, Mengmeng,Li, Qian,Liang, Le,et al. Real-time visualization of clustering and intracellular transport of gold nanoparticles by correlative imaging[J]. NATURE COMMUNICATIONS,2017,8(-):15646. |
| APA | Liu, Mengmeng.,Li, Qian.,Liang, Le.,Li, Jiang.,Wang, Kun.,...&,.(2017).Real-time visualization of clustering and intracellular transport of gold nanoparticles by correlative imaging.NATURE COMMUNICATIONS,8(-),15646. |
| MLA | Liu, Mengmeng,et al."Real-time visualization of clustering and intracellular transport of gold nanoparticles by correlative imaging".NATURE COMMUNICATIONS 8.-(2017):15646. |
入库方式： OAI收割
来源：上海营养与健康研究所
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