Role of influenza A virus NP acetylation on viral growth and replication
文献类型：期刊论文
| 作者 | Giese, Sebastian2,3; Ciminski, Kevin2,3; Bolte, Hardin2,3; Moreira, Etori Aguiar2,3,4,5; David, Quinnlan2,3; Goetz, Veronika2,3; Schwemmle, Martin2,3; Bolte, Hardin4,5; Lakdawala, Seema6; Hu, Zehan7,8 |
| 刊名 | NATURE COMMUNICATIONS
 |
| 出版日期 | 2017 |
| 卷号 | 8期号:-页码:1259 |
| ISSN号 | 2041-1723 |
| 关键词 | JNK NF-kappa B non-alcoholic fatty liver disease periodontitis Porphyromonas gingivalis |
| DOI | 10.1038/s41467-017-01112-3 |
| 文献子类 | Article |
| 英文摘要 | Lysine acetylation is a post-translational modification known to regulate protein functions. Here we identify several acetylation sites of the influenza A virus nucleoprotein (NP), including the lysine residues K77, K113 and K229. Viral growth of mutant virus encoding K229R, mimicking a non-acetylated NP lysine residue, is severely impaired compared to wildtype or the mutant viruses encoding K77R or K113R. This attenuation is not the result of decreased polymerase activity, altered protein expression or disordered vRNP co-segregation but rather caused by impaired particle release. Interestingly, release deficiency is also observed mimicking constant acetylation at this site (K229Q), whereas virus encoding NP-K113Q could not be generated. However, mimicking NP hyper-acetylation at K77 and K229 severely diminishes viral polymerase activity, while mimicking NP hypo-acetylation at these sites has no effect on viral replication. These results suggest that NP acetylation at K77, K113 and K229 impacts multiple steps in viral replication of influenza A viruses. |
| 学科主题 | Science & Technology - Other Topics |
| WOS关键词 | ANTIVIRAL DRUG DEVELOPMENT ; NUCLEAR EXPORT PROTEIN ; CRYSTAL-STRUCTURE ; RNA-BINDING ; HISTONE H3 ; NUCLEOPROTEIN ; RIBONUCLEOPROTEINS ; PHOSPHORYLATION ; UBIQUITINATION ; CHROMOSOME |
| 语种 | 英语 |
| 出版者 | NATURE PUBLISHING GROUP |
| WOS记录号 | WOS:000414245000005 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/1086]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所 |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Hlth Sci, Shanghai 200031, Peoples R China; 2.Univ Freiburg, Inst Virol, Med Ctr, D-79104 Freiburg, Germany; 3.Univ Freiburg, Fac Med, D-79104 Freiburg, Germany; 4.Univ Freiburg, Spemann Grad Sch Biol & Med, D-79104 Freiburg, Germany; 5.Univ Freiburg, Fac Biol, D-79104 Freiburg, Germany; 6.Univ Pittsburgh, Dept Microbiol & Mol Genet, Sch Med, Pittsburgh, PA 15217 USA; 7.Univ Freiburg, Dept Dermatol, Med Ctr, D-79110 Freiburg, Germany; 8.Univ Fribourg, Dept Biol, CH-79110 Fribourg, Switzerland; 9.Philipps Univ Marburg, Inst Virol, D-35043 Marburg, Germany; 10.Chinese Acad Sci, Inst Pasteur Shanghai, Shanghai Inst Biol Sci, Key Lab Mol Virol & Immunol, Shanghai 200031, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Giese, Sebastian,Ciminski, Kevin,Bolte, Hardin,et al. Role of influenza A virus NP acetylation on viral growth and replication[J]. NATURE COMMUNICATIONS,2017,8(-):1259. |
| APA | Giese, Sebastian.,Ciminski, Kevin.,Bolte, Hardin.,Moreira, Etori Aguiar.,David, Quinnlan.,...&,.(2017).Role of influenza A virus NP acetylation on viral growth and replication.NATURE COMMUNICATIONS,8(-),1259. |
| MLA | Giese, Sebastian,et al."Role of influenza A virus NP acetylation on viral growth and replication".NATURE COMMUNICATIONS 8.-(2017):1259. |
入库方式： OAI收割
来源：上海营养与健康研究所
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