SPOP-containing complex regulates SETD2 stability and H3K36me3-coupled alternative splicing
文献类型：期刊论文
| 作者 | Zhu, Kun2; Lei, Pin-Ji2; Ju, Lin-Gao2; Wang, Xiang2; Yang, Bo2; Shao, Changwei2; Zhu, Yuan2; Fu, Xiang-Dong2; Li, Lianyun2; Wu, Min2 |
| 刊名 | NUCLEIC ACIDS RESEARCH
 |
| 出版日期 | 2017 |
| 卷号 | 45期号:1页码:92-105 |
| 关键词 | chemoresistance mitogen-activated protein kinase pancreatic cancer perineural invasion |
| ISSN号 | 0305-1048 |
| DOI | 10.1093/nar/gkw814 |
| 文献子类 | Article |
| 英文摘要 | Trimethylation of histone H3K36 is a chromatin mark associated with active gene expression, which has been implicated in coupling transcription with mRNA splicing and DNA damage response. SETD2 is a major H3K36 trimethyltransferase, which has been implicated as a tumor suppressor in mammals. Here, we report the regulation of SETD2 protein stability by the proteasome system, and the identification of SPOP, a key subunit of the CUL3 ubiquitin E3 ligase complex, as a SETD2-interacting protein. We demonstrate that SPOP is critically involved in SETD2 stability control and that the SPOP/CUL3 complex is responsible for SETD2 polyubiquitination both in vivo and in vitro. ChIP-Seq analysis and biochemical experiments demonstrate that modulation of SPOP expression confers differential H3K36me3 on SETD2 target genes, and induce H3K36me3-coupled alternative splicing events. Together, these findings establish a functional connection between oncogenic SPOP and tumor suppressive SETD2 in the dynamic regulation of gene expression on chromatin. |
| 学科主题 | Biochemistry & Molecular Biology |
| WOS关键词 | HISTONE H3K36 METHYLATION ; H3 LYSINE 36 ; PROSTATE-CANCER ; KIDNEY CANCER ; HOMOLOGOUS RECOMBINATION ; UBIQUITIN LIGASE ; RENAL-CARCINOMA ; GENETIC-BASIS ; TRIMETHYLATION ; PROTEIN |
| 语种 | 英语 |
| WOS记录号 | WOS:000396575100013 |
| 出版者 | OXFORD UNIV PRESS |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/1093]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所 |
| 作者单位 | 1.Univ Calif San Diego, Dept Cellular & Mol Med, La Jolla, CA 92093 USA; 2.Wuhan Univ, Hubei Key Lab Cell Homeostasis, Hubei Key Lab Devel Originated Dis, Dept Biochem & Mol Biol,Coll Life Sci, Wuhan 430072, Hubei, Peoples R China; 3.Univ Texas Southwestern Med Ctr Dallas, Dept Dev Biol, Dallas, TX 75390 USA; 4.Chinese Acad Sci, Shanghai Inst Biol Sci, CAS MPG Partner Inst Computat Biol, Shanghai 200031, Peoples R China; 5.Univ Calif San Diego, Inst Genom Med, La Jolla, CA 92093 USA, |
| 推荐引用方式 GB/T 7714 | Zhu, Kun,Lei, Pin-Ji,Ju, Lin-Gao,et al. SPOP-containing complex regulates SETD2 stability and H3K36me3-coupled alternative splicing[J]. NUCLEIC ACIDS RESEARCH,2017,45(1):92-105. |
| APA | Zhu, Kun.,Lei, Pin-Ji.,Ju, Lin-Gao.,Wang, Xiang.,Yang, Bo.,...&,.(2017).SPOP-containing complex regulates SETD2 stability and H3K36me3-coupled alternative splicing.NUCLEIC ACIDS RESEARCH,45(1),92-105. |
| MLA | Zhu, Kun,et al."SPOP-containing complex regulates SETD2 stability and H3K36me3-coupled alternative splicing".NUCLEIC ACIDS RESEARCH 45.1(2017):92-105. |
入库方式： OAI收割
来源：上海营养与健康研究所
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