The spontaneous differentiation and chromosome loss in iPSCs of human trisomy 18 syndrome
文献类型：期刊论文
| 作者 | Li, Ting2; Han, Xu2; Guo, Ying2; Lai, Dongmei2; Zhao, Hanzhi3; Zhang, Lingling3; Jin, Ying3; Yao, Jiaying1; Shao, Zhen1; , |
| 刊名 | CELL DEATH & DISEASE
 |
| 出版日期 | 2017 |
| 卷号 | 8期号:-页码:e3149 |
| 关键词 | arterial pressure blood pressure epidemiology genome-wide association study |
| ISSN号 | 2041-4889 |
| DOI | 10.1038/cddis.2017.565 |
| 文献子类 | Article |
| 英文摘要 | Aneuploidy including trisomy results in developmental disabilities and is the leading cause of miscarriages in humans. Unlike trisomy 21, pathogenic mechanisms of trisomy 18 remain unclear. Here, we successfully generated induced pluripotent stem cells (iPSCs) from human amniotic fluid cells (AFCs) with trisomy 18 pregnancies. We found that trisomy 18 iPSCs (18T-iPSCs) were prone to differentiate spontaneously. Intriguingly, 18T-iPSCs lost their extra 18 chromosomes and converted to diploid cells after 10 generations. fluorescence in situ hybridization analysis showed chromosome loss was a random event that might happen in any trisomic cells. Selection undifferentiated cells for passage accelerated the recovery of euploid cells. Overall, our findings indicate the genomic instability of trisomy 18 iPSCs bearing an extra chromosome 18. |
| 学科主题 | Cell Biology |
| WOS关键词 | EMBRYONIC STEM-CELLS ; DOWN-SYNDROME ; GENOMIC INSTABILITY ; ANEUPLOIDY ; TRISOMY-21 ; YEAST |
| 语种 | 英语 |
| WOS记录号 | WOS:000414022900010 |
| 出版者 | NATURE PUBLISHING GROUP |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/1124]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所 |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Partner Inst Computat Biol, Shanghai 200031, Peoples R China, 2.Shanghai Jiao Tong Univ, Sch Med, Int Peace Matern & Child Hlth Hosp, 145 Guangyuan Rd, Shanghai 200030, Peoples R China; 3.Shanghai Jiao Tong Univ, Univ Chinese Acad Sci,Key Lab Stem Cell Biol, Chinese Acad Sci,CAS Ctr Excellence Mol Cell Sci, Sch Med,Inst Hlth Sci,Shanghai Inst Biol Sci, 320 Yueyang Rd, Shanghai 200032, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Li, Ting,Han, Xu,Guo, Ying,et al. The spontaneous differentiation and chromosome loss in iPSCs of human trisomy 18 syndrome[J]. CELL DEATH & DISEASE,2017,8(-):e3149. |
| APA | Li, Ting.,Han, Xu.,Guo, Ying.,Lai, Dongmei.,Zhao, Hanzhi.,...&,.(2017).The spontaneous differentiation and chromosome loss in iPSCs of human trisomy 18 syndrome.CELL DEATH & DISEASE,8(-),e3149. |
| MLA | Li, Ting,et al."The spontaneous differentiation and chromosome loss in iPSCs of human trisomy 18 syndrome".CELL DEATH & DISEASE 8.-(2017):e3149. |
入库方式： OAI收割
来源：上海营养与健康研究所
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