lncRNA ADAMTS9-AS2 Controls Human Mesenchymal Stem Cell Chondrogenic Differentiation and Functions as a ceRNA
文献类型：期刊论文
| 作者 | Huang, Ming-Jian1; Zhao, Jing-Yu1; Zhang, Xiao-Ling1; Xu, Jia-Jia2,3; Li, Jing2,3; Zhuang, Yi-Fu4; , |
| 刊名 | MOLECULAR THERAPY-NUCLEIC ACIDS
 |
| 出版日期 | 2019 |
| 卷号 | 18期号:-页码:533-545 |
| 关键词 | Retro-transposable hotspot BMP2 Fat tail Sheep Evolution |
| ISSN号 | 2162-2531 |
| DOI | 10.1016/j.omtn.2019.08.027 |
| 文献子类 | Article |
| 英文摘要 | Long noncoding RNAs (lncRNAs) have emerged as key regulators of cell differentiation and development. However, potential roles for lncRNAs in chondrogenic differentiation have remained poorly understood. Here we identify lncRNA ADAMTS9 antisense RNA 2, ADAMTS9-AS2, which controls the chondrogenic differentiation by acting as a competing endogenous RNA (ceRNA) in human mesenchymal stem cells (hMSCs). We screen out ADAMTS9-AS2 of undifferentiated and differentiated cells during chondrogenic differentiation by microarrays. Suppression or overexpression of lncRNA ADAMTS9-AS2 correlates with inhibition and promotion of hMSC chondrogenic differentiation, respectively. We find that ADAMTS9-AS2 can sponge miR-942-5p to regulate the expression of Scrg1, a transcription factor promoting chondrogenic gene expression. Finally, we confirm the function of ADAMTS9-AS2 to cartilage repair in the absence of transforming growth factor beta (TGF-beta) in vivo. In conclusion, ADAMTS9-AS2 plays an important role in chondrogenic differentiation as a ceRNA, so that it can be regarded as a therapy target for cartilage repair. |
| 学科主题 | Cell Biology |
| WOS关键词 | LONG NONCODING RNA ; TGF-BETA ; GROWTH ; MECHANISMS ; EXPRESSION ; CULTURE ; MODEL ; GENE |
| 语种 | 英语 |
| CSCD记录号 | CSCD:31671346 |
| WOS记录号 | WOS:000500716400048 |
| 出版者 | CELL PRESS |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/1133]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所 |
| 作者单位 | 1.Shanghai Jiao Tong Univ, Sch Med, Xinhua Hosp, Dept Orthoped Surg, Room 323,Sci Res Ctr Phase 2,768 Shuangliao Rd, Shanghai 200092, Peoples R China; 2.SJTUSM, Inst Hlth Sci, Key Lab Stem Cell Biol, Shanghai 200031, Peoples R China; 3.Chinese Acad Sci, SIBS, Shanghai 200031, Peoples R China; 4.SJTUSM, Shanghai Peoples Hosp 9, Dept Orthoped Surg, Shanghai 201999, Peoples R China, |
| 推荐引用方式 GB/T 7714 | Huang, Ming-Jian,Zhao, Jing-Yu,Zhang, Xiao-Ling,et al. lncRNA ADAMTS9-AS2 Controls Human Mesenchymal Stem Cell Chondrogenic Differentiation and Functions as a ceRNA[J]. MOLECULAR THERAPY-NUCLEIC ACIDS,2019,18(-):533-545. |
| APA | Huang, Ming-Jian.,Zhao, Jing-Yu.,Zhang, Xiao-Ling.,Xu, Jia-Jia.,Li, Jing.,...&,.(2019).lncRNA ADAMTS9-AS2 Controls Human Mesenchymal Stem Cell Chondrogenic Differentiation and Functions as a ceRNA.MOLECULAR THERAPY-NUCLEIC ACIDS,18(-),533-545. |
| MLA | Huang, Ming-Jian,et al."lncRNA ADAMTS9-AS2 Controls Human Mesenchymal Stem Cell Chondrogenic Differentiation and Functions as a ceRNA".MOLECULAR THERAPY-NUCLEIC ACIDS 18.-(2019):533-545. |
入库方式： OAI收割
来源：上海营养与健康研究所
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