Nascent Pre-rRNA Sorting via Phase Separation Drives the Assembly of Dense Fibrillar Components in the Human Nucleolus
文献类型：期刊论文
| 作者 | Yao, Run-Wen2; Xu, Guang2; Shan, Lin2; Luan, Peng-Fei2; Wang, Yang2; Wu, Man2; Yang, Liang-Zhong2; Xing, Yu-Hang2; Chen, Ling-Ling2,3; Wang, Ying1 |
| 刊名 | MOLECULAR CELL
 |
| 出版日期 | 2019 |
| 卷号 | 76期号:5页码:767+ |
| 关键词 | carbohydrates cardiovascular disease chronic disease diet fatty acids |
| ISSN号 | 1097-2765 |
| DOI | 10.1016/j.molcel.2019.08.014 |
| 文献子类 | Article |
| 英文摘要 | Fibrillar centers (FCs) and dense fibrillar components (DFCs) are essential morphologically distinct sub-regions of mammalian cell nucleoli for rDNA transcription and pre-rRNA processing. Here, we report that a human nucleolus consists of several dozen FC/DFC units, each containing 2-3 transcriptionally active rDNAs at the FC/DFC border. Pre-rRNA processing factors, such as fibrillarin (FBL), form 18-24 clusters that further assemble into the DFC surrounding the FC. Mechanistically, the 5' end of nascent 47S pre-rRNA binds co-transcriptionally to the RNA-binding domain of FBL. FBL diffuses to the DFC, where local self-association via its glycine- and arginine-rich (GAR) domain forms phase-separated clusters to immobilize FBL-interacting pre-rRNA, thus promoting directional traffic of nascent pre-rRNA while facilitating pre-rRNA processing and DFC formation. These results unveil FC/DFC ultrastructures in nucleoli and suggest a conceptual framework for considering nascent RNA sorting using multivalent interactions of their binding proteins. |
| 学科主题 | Hematology |
| WOS关键词 | POLYMERASE-I TRANSCRIPTION ; QUANTITATIVE-ANALYSIS ; ORGANIZER REGIONS ; SSU PROCESSOME ; BOX C/D ; BINDING ; HETEROCHROMATIN ; PROTEINS ; GENES ; ACTIVATION |
| 语种 | 英语 |
| CSCD记录号 | CSCD:31540874 |
| WOS记录号 | WOS:000500937100009 |
| 出版者 | CELL PRESS |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/1137]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所 |
| 作者单位 | 1.Chinese Acad Sci, Univ Chinese Acad Sci, Shanghai Inst Nutr & Hlth,Shanghai Inst Biol Sci, CAS Key Lab Computat Biol,CAS MPG Partner Inst Co, 320 Yueyang Rd, Shanghai 200031, Peoples R China; 2.Chinese Acad Sci, Univ Chinese Acad Sci, State Key Lab Mol Biol,Shanghai Key Lab Mol Andro, CAS Ctr Excellence Mol Cell Sci,Shanghai Inst Bio, 320 Yueyang Rd, Shanghai 200031, Peoples R China; 3.ShanghaiTech Univ, Sch Life Sci & Technol, 100 Haike Rd, Shanghai 201210, Peoples R China; 4.Massachusetts Gen Hosp, Dept Pathol, Boston, MA 02114 USA; 5.Harvard Med Sch, Boston, MA 02114 USA, |
| 推荐引用方式 GB/T 7714 | Yao, Run-Wen,Xu, Guang,Shan, Lin,et al. Nascent Pre-rRNA Sorting via Phase Separation Drives the Assembly of Dense Fibrillar Components in the Human Nucleolus[J]. MOLECULAR CELL,2019,76(5):767+. |
| APA | Yao, Run-Wen.,Xu, Guang.,Shan, Lin.,Luan, Peng-Fei.,Wang, Yang.,...&,.(2019).Nascent Pre-rRNA Sorting via Phase Separation Drives the Assembly of Dense Fibrillar Components in the Human Nucleolus.MOLECULAR CELL,76(5),767+. |
| MLA | Yao, Run-Wen,et al."Nascent Pre-rRNA Sorting via Phase Separation Drives the Assembly of Dense Fibrillar Components in the Human Nucleolus".MOLECULAR CELL 76.5(2019):767+. |
入库方式： OAI收割
来源：上海营养与健康研究所
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