Transplantation of betatrophin-expressing adipose-derived mesenchymal stem cells induces -cell proliferation in diabetic mice
文献类型：期刊论文
| 作者 | Sun, Liang-Liang2; Tang, Wei2; Zou, Jun-Jie2; Chen, Xiang-Fang2; Zheng, Jiao-Yang2; Shi, Yong-Quan2; Jiang, Bei-Ge3; Liu, Tian-Jin4; Li, Limei1; , |
| 刊名 | INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
 |
| 出版日期 | 2017 |
| 卷号 | 39期号:4页码:936-948 |
| 关键词 | adipose-derived mesenchymal stem cells betatrophin islets streptozotocin-induced diabetes transplantation |
| ISSN号 | 1107-3756 |
| DOI | 10.3892/ijmm.2017.2914 |
| 文献子类 | Article |
| 英文摘要 | Recent progress in regenerative medicine has suggested that mesenchymal stem cell (MSC)-based therapy is a novel potential cure for diabetes. Betatrophin is a newly identified hormone that can increase the production and expansion of insulin-secreting -cells when administered to mice. In this study, we evaluated the effect of betatrophin overexpression by human adipose-derived MSCs (ADMSCs) by in vitro experiments, as well as following their transplantation into a mice with streptozotocin (STZ)-induced diabetes. The overexpression of betatrophin did not affect the ADMSCs in terms of proliferation, differentiation and morphology. However, the co-culture of human islets with ADMSCs overexpressing betatrophin (ADMSCs-BET) induced islet proliferation, -cell specific transcription factor expression, and the islet production of insulin under the stimulation of glucose or KCl and Arg. In addition, ADMSCs-BET enhanced the anti-inflammatory and anti-apoptotic effects of the co-cultured islets compared with ADMSCs cultured alone. In mice with STZ-induced diabetes, the transplantation of ADMSCs-BET ameliorated the hyperglycemia and weight loss associated with STZ-induced diabetes; ADMSCs-BET also significantly enhanced the ratio of -cells per islet compared to the transplantation of ADMSCs alone. Thus, our study demonstrates a novel strategy for inducing -cell regeneration. ADMSCs-BET may replace insulin injections by increasing the number of endogenous insulin-producing cells in patients with diabetes. This combined strategy of ADMSC transplantation and gene therapy may prove to be a useful therapy for the treatment of diabetes. |
| 学科主题 | Research & Experimental Medicine |
| WOS关键词 | INSULIN-PRODUCING CELLS ; ANGIOPOIETIN-LIKE PROTEIN ; STROMAL CELLS ; BONE-MARROW ; IN-VITRO ; ALPHA-CELLS ; BETA-CELLS ; DIFFERENTIATION ; GENE ; IMMUNOMODULATION |
| 语种 | 英语 |
| WOS记录号 | WOS:000399915100018 |
| 出版者 | SPANDIDOS PUBL LTD |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/1139]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所 |
| 作者单位 | 1.Shanghai East Hosp, Minist Educ, Key Lab Arrhythmias, Clin & Translat Res Ctr, Shanghai 200120, Peoples R China, 2.Second Mil Med Univ, Changzheng Hosp, Dept Endocrinol, 415 Rd Fengyang, Shanghai 200003, Peoples R China; 3.Second Mil Med Univ, Eastern Hepatobiliary Surg Hosp, Dept Hepat Surg, 225 Rd Changhai, Shanghai 200438, Peoples R China; 4.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Inst Hlth Sci, Shanghai 200031, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Sun, Liang-Liang,Tang, Wei,Zou, Jun-Jie,et al. Transplantation of betatrophin-expressing adipose-derived mesenchymal stem cells induces -cell proliferation in diabetic mice[J]. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE,2017,39(4):936-948. |
| APA | Sun, Liang-Liang.,Tang, Wei.,Zou, Jun-Jie.,Chen, Xiang-Fang.,Zheng, Jiao-Yang.,...&,.(2017).Transplantation of betatrophin-expressing adipose-derived mesenchymal stem cells induces -cell proliferation in diabetic mice.INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE,39(4),936-948. |
| MLA | Sun, Liang-Liang,et al."Transplantation of betatrophin-expressing adipose-derived mesenchymal stem cells induces -cell proliferation in diabetic mice".INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE 39.4(2017):936-948. |
入库方式： OAI收割
来源：上海营养与健康研究所
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