Identification of three selenoprotein T paralogs in goldfish (Carassius auratus) and expression analysis in response to environmental stressors
文献类型：期刊论文
| 作者 | Chen, Wenbo1; Zhang, Zhen1; Yan, Fangfang1; Jiang, Xiaoxue1; Qin, Shaozong1; Dong, Haiyan2; Jiang, Xiaoxue3; , |
| 刊名 | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
 |
| 出版日期 | 2017 |
| 卷号 | 203期号:-页码:65-75 |
| 关键词 | Carassius auratus Selenoprotein T mRNA expression Cadmium Oxidative stress Heat stress |
| ISSN号 | 1096-4959 |
| DOI | 10.1016/j.cbpb.2016.09.004 |
| 文献子类 | Article |
| 英文摘要 | Selenoproteins, which contain the selenocysteine (Sec), play crucial roles in antioxidant protection. In the present study, we have identified and characterized three selenoprotein T paralogs (designated as gfSelT1a, gfSelT1b and gfSelT2) in goldfish, Carassius auratus. The SECIS element was found in the 3'-UTRs of gfSelT1a, gfSelT1b and gfSelT2 mRNA. Sequence analysis showed that they all possess the CxxU motif (where U represents Sec) in the N-terminal. Constitutive expressions of gfSelT1a, gfSelT1b and gfSelT2 were observed in all tissues studied. In liver, gfSelT1a, gfSelT1b and gfSelT2 mRNA expression levels significantly decreased when fasting and increased after re-feeding. The expressions of gfSelT1a, gfSelT1b and gfSelT2 were all inducible by cadmium exposure and H2O2-mediated oxidative stress, except the transcripts of gfSelT1b decreasing with H2O2 stimulation in brain. Furthermore, the expressions of gfSelT1a, gfSelT1b and gfSelT2 consistently increased in spleen but decreased in brain in response to heat stress. However, in liver only gfSelT1a mRNA expression significantly increased. In a word, this is the first report of the presence of three SelT genes in goldfish and their distinct expression patterns in response to environmental stressors. Our results suggest a potential involvement of gfSelT1a, gfSelT1b and gfSelT2 in host protection against environmental stressors including heavy mental, oxidative stress and heat stress in goldfish. (C) 2016 Elsevier Inc. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology ; Zoology |
| WOS关键词 | CADMIUM EXPOSURE ; MESSENGER-RNA ; HEAT-STRESS ; SELENIUM ; GENES ; PROTEIN ; ANTIOXIDANT ; METABOLISM ; ELEMENTS ; CELLS |
| 语种 | 英语 |
| WOS记录号 | WOS:000390626800008 |
| 出版者 | ELSEVIER SCIENCE INC |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/1161]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所 |
| 作者单位 | 1.Henan Polytech Univ, Dept Biol, Inst Resources & Environm, Jiaozuo 454000, Henan, Peoples R China; 2.Huzhou Univ, Dept Basic Med Sci, Huzhou 313000, Zhejiang, Peoples R China; 3.Chinese Acad Sci, Shanghai Inst Biol Sci, Grad Sch, Key Lab Nutr & Metab,Inst Nutr Sci, Shanghai 200031, Peoples R China, |
| 推荐引用方式 GB/T 7714 | Chen, Wenbo,Zhang, Zhen,Yan, Fangfang,et al. Identification of three selenoprotein T paralogs in goldfish (Carassius auratus) and expression analysis in response to environmental stressors[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2017,203(-):65-75. |
| APA | Chen, Wenbo.,Zhang, Zhen.,Yan, Fangfang.,Jiang, Xiaoxue.,Qin, Shaozong.,...&,.(2017).Identification of three selenoprotein T paralogs in goldfish (Carassius auratus) and expression analysis in response to environmental stressors.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,203(-),65-75. |
| MLA | Chen, Wenbo,et al."Identification of three selenoprotein T paralogs in goldfish (Carassius auratus) and expression analysis in response to environmental stressors".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 203.-(2017):65-75. |
入库方式： OAI收割
来源：上海营养与健康研究所
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