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Interactions of human embryonic stem cell-derived cardiovascular progenitor cells with immobilized extracellular matrix proteins

文献类型：期刊论文

作者Lu, Jizhen1,2,3; Huang, Jijun1,2,3; Liu, Qiao1,2,3; Zhang, Peng1,2,3; Gao, Ling1,2,3; Yang, Huang-Tian1,2,3; Kaestle, Katrin4; Gardiner, James4; Thissen, Helmut4; ,
刊名JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
出版日期2017
卷号105期号:4页码:1094-1104
关键词cardiovascular progenitor cells extracellular matrix components surface modification biointerfacial interactions cell attachment and survival
ISSN号1549-3296
DOI10.1002/jbm.a.36005
文献子类Article
英文摘要Human embryonic stem cell-derived cardiovascular progenitor cells (hESC-CVPCs) hold great promise for cell-based therapies of heart diseases. However, little is known about their niche microenvironment and in particular the required extracellular matrix (ECM) components. Here we screened combinations of surface-immobilized ECM proteins to identify substrates that support the attachment and survival of hESC-CVPCs. Covalent immobilization of ECM proteins laminin (Lm), fibronectin (Fn), collagen I (CI), collagen III (CIII), and collagen IV (CIV) in multiple combinations and concentrations was achieved by reductive amination on transparent acetaldehyde plasma polymer (AAPP) interlayer coatings. We identified that CI, CIII, CIV, and Fn and their combinations were important for hESC-CVPC attachment and survival, while Lm was dispensable. Moreover, for coatings displaying single ECM proteins, CI and CIII performed better than CIV and Fn, while coatings displaying the combined ECM proteins CIII+CIV and Fn+CIII+CIV at 100 mu g/mL were comparable to Matrigel in regard to supporting hESC-CVPC attachment and viability. Our results identify ECM proteins required for hESC-CVPCs and demonstrate that coatings displaying multiple immobilized ECM proteins offer a suitable microenvironment for the attachment and survival of hESC-CVPCs. This knowledge contributes to the development of approaches for maintaining hESC-CVPCs and therefore to advances in cardiovascular regeneration. (C) 2017 Wiley Periodicals, Inc.
学科主题Engineering ; Materials Science
WOS关键词RANDOMIZED PHASE-1 TRIAL ; CARDIOMYOCYTE DIFFERENTIATION ; ISCHEMIC CARDIOMYOPATHY ; CARDIAC DIFFERENTIATION ; MYOCARDIAL-INFARCTION ; SURFACES ; COATINGS ; HEART ; POPULATION ; GROWTH
语种英语
WOS记录号WOS:000395008300014
出版者WILEY
版本出版稿
源URL[http://202.127.25.144/handle/331004/1169]  
专题中国科学院上海生命科学研究院营养科学研究所
作者单位1.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Hlth Sci, Key Lab Stem Cell Biol, Biol Res Bldg A,320 Yueyang Rd, Shanghai 200031, Peoples R China;
2.Shanghai Jiao Tong Univ, Sch Med, Biol Res Bldg A,320 Yueyang Rd, Shanghai 200031, Peoples R China;
3.Zhejiang Univ, Affiliated Hosp 2, 88 Jiefang Rd, Hangzhou 310009, Zhejiang, Peoples R China;
4.CSIRO Mfg, Bayview Ave, Clayton, Vic 3168, Australia,
推荐引用方式
GB/T 7714		 Lu, Jizhen,Huang, Jijun,Liu, Qiao,et al. Interactions of human embryonic stem cell-derived cardiovascular progenitor cells with immobilized extracellular matrix proteins[J]. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A,2017,105(4):1094-1104.
APA Lu, Jizhen.,Huang, Jijun.,Liu, Qiao.,Zhang, Peng.,Gao, Ling.,...&,.(2017).Interactions of human embryonic stem cell-derived cardiovascular progenitor cells with immobilized extracellular matrix proteins.JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A,105(4),1094-1104.
MLA Lu, Jizhen,et al."Interactions of human embryonic stem cell-derived cardiovascular progenitor cells with immobilized extracellular matrix proteins".JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A 105.4(2017):1094-1104.

入库方式： OAI收割

来源：上海营养与健康研究所

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