Independent manipulation of histone H3 modifications in individual nucleosomes reveals the contributions of sister histones to transcription
文献类型：期刊论文
| 作者 | Zhou, Zhen1; Liu, Yu-Ting1; Gong, Ting1; Li, Hong-Tao1; Cai, Chen1; Zhang, Ling-Li1; Zhou, Jin-Qiu1,3; Ma, Li2; Hu, Ya-Nan2; Wei, Gang2 |
| 刊名 | ELIFE
 |
| 出版日期 | 2017 |
| 卷号 | 6期号:-页码:e30178 |
| 关键词 | adipogenesis CCAAT-enhancer-binding protein (C EBP) nuclear receptor peroxisome proliferator-activated receptor (PPAR) transcription corepressor |
| ISSN号 | 2050-084X |
| DOI | 10.7554/eLife.30178 |
| 文献子类 | Article |
| 英文摘要 | Histone tail modifications can greatly influence chromatin-associated processes. Asymmetrically modified nucleosomes exist in multiple cell types, but whether modifications on both sister histones contribute equally to chromatin dynamics remains elusive. Here, we devised a bivalent nucleosome system that allowed for the constitutive assembly of asymmetrically modified sister histone H3s in nucleosomes in Saccharomyces cerevisiae. The sister H3K36 methylations independently affected cryptic transcription in gene coding regions, whereas sister H3K79 methylation had cooperative effects on gene silencing near telomeres. H3K4 methylation on sister histones played an independent role in suppressing the recruitment of Gal4 activator to the GAL1 promoter and in inhibiting GAL1 transcription. Under starvation stress, sister H3K4 methylations acted cooperatively, independently or redundantly to regulate transcription. Thus, we provide a unique tool for comparing symmetrical and asymmetrical modifications of sister histone H3s in vivo. |
| 学科主题 | Life Sciences & Biomedicine - Other Topics |
| WOS关键词 | ASYMMETRICALLY MODIFIED NUCLEOSOMES ; LARGE GENE LISTS ; SACCHAROMYCES-CEREVISIAE ; CHROMATIN-STRUCTURE ; CORE PARTICLE ; LYSINE 4 ; CRYSTAL-STRUCTURE ; CODING REGIONS ; METHYLATION ; YEAST |
| 语种 | 英语 |
| WOS记录号 | WOS:000414742000001 |
| 出版者 | ELIFE SCIENCES PUBLICATIONS LTD |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/1173]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所 |
| 作者单位 | 1.Univ Chinese Acad Sci, Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol,CAS Ctr Excelle, Innovat Ctr Cell Signaling Network,State Key Lab, Shanghai, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Biol Sci, CAS MPG Partner Inst Computat Biol, Key Lab Computat Biol, Shanghai, Peoples R China; 3.Shanghai Tech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China, |
| 推荐引用方式 GB/T 7714 | Zhou, Zhen,Liu, Yu-Ting,Gong, Ting,et al. Independent manipulation of histone H3 modifications in individual nucleosomes reveals the contributions of sister histones to transcription[J]. ELIFE,2017,6(-):e30178. |
| APA | Zhou, Zhen.,Liu, Yu-Ting.,Gong, Ting.,Li, Hong-Tao.,Cai, Chen.,...&,.(2017).Independent manipulation of histone H3 modifications in individual nucleosomes reveals the contributions of sister histones to transcription.ELIFE,6(-),e30178. |
| MLA | Zhou, Zhen,et al."Independent manipulation of histone H3 modifications in individual nucleosomes reveals the contributions of sister histones to transcription".ELIFE 6.-(2017):e30178. |
入库方式： OAI收割
来源：上海营养与健康研究所
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