Lineage conversion of mouse fibroblasts to pancreatic alpha-cells
文献类型：期刊论文
| 作者 | Liu, Tianjin2; Cen, Jin2; Chen, Xiaotao2; Cheng, Xin2; Hui, Lijian2; Sun, Liangliang3; Shi, Yongquan3; Jiang, Beige4; Li, Limei5,6; Zhang, Zhaoyun1 |
| 刊名 | EXPERIMENTAL AND MOLECULAR MEDICINE
 |
| 出版日期 | 2017 |
| 卷号 | 49期号:-页码:e350 |
| ISSN号 | 1226-3613 |
| 关键词 | DP1 receptor niacin prostaglandin retention enema ulcerative colitis |
| DOI | 10.1038/emm.2017.84 |
| 文献子类 | Article |
| 英文摘要 | alpha-cells, which synthesize glucagon, also support beta-cell survival and have the capacity to transdifferentiate into beta-cells. However, the role of alpha-cells in pathological conditions and their putative clinical applications remain elusive due in large part to the lack of mature alpha-cells. Here, we present a new technique to generate functional a-like cells. alpha-like cells (iAlpha cells) were generated from mouse fibroblasts by transduction of transcription factors, including Hhex, Foxa3, Gata4, Pdx1 and Pax4, which induce alpha-cell-specific gene expression and glucagon secretion in response to KCl and Arg stimulation. The cell functions in vivo and in vitro were evaluated. Lineage-specific and functional-related gene expression was tested by realtime PCR, insulin tolerance test (ITT), glucose tolerance test (GTT), Ki67 and glucagon immunohistochemistry analysis were done in iAlpha cells transplanted nude mice. iAlpha cells possess alpha-cell function in vitro and alter blood glucose levels in vivo. Transplantation of iAlpha cells into nude mice resulted in insulin resistance and increased beta-cell proliferation. Taken together, we present a novel strategy to generate functional alpha-like cells for the purposes of disease modeling and regenerative medicine. |
| 学科主题 | Biochemistry & Molecular Biology ; Research & Experimental Medicine |
| WOS关键词 | EMBRYONIC STEM-CELLS ; BETA-CELL ; IN-VIVO ; IMMUNOSUPPRESSIVE REGIMEN ; ISLET TRANSPLANTATION ; DIABETES-MELLITUS ; ENDOCRINE-CELLS ; DEFINED FACTORS ; INSULIN ; EXPRESSION |
| 语种 | 英语 |
| 出版者 | NATURE PUBLISHING GROUP |
| WOS记录号 | WOS:000405988200002 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/1206]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所 |
| 作者单位 | 1.Huashan Hosp, Dept Endocrinol & Metab, Shanghai, Peoples R China; 2.Chinese Acad Sci, Inst Biochem & Cell Biol, Inst Hlth Sci, Shanghai Inst Biol Sci, 320 Rd Yueyang, Shanghai 200031, Peoples R China; 3.Second Mil Med Univ, Dept Endocrinol & Metab, Changzheng Hosp, Shanghai, Peoples R China; 4.Second Mil Med Univ, Dept Hepat Surg, Eastern Hepatobiliary Surg Hosp, Shanghai, Peoples R China; 5.Tongji Univ, Sch Med, Res Ctr Translat Med, Shanghai East Hosp, Shanghai, Peoples R China; 6.Tongji Univ, Sch Med, Dept Vasc Surg, Shanghai East Hosp, Shanghai, Peoples R China; 7.St Michaels Hosp, Div Endocrinol & Metab, Keenan Res Ctr, Li Ka Shing Knowledge Inst, Toronto, ON, Canada, |
| 推荐引用方式 GB/T 7714 | Liu, Tianjin,Cen, Jin,Chen, Xiaotao,et al. Lineage conversion of mouse fibroblasts to pancreatic alpha-cells[J]. EXPERIMENTAL AND MOLECULAR MEDICINE,2017,49(-):e350. |
| APA | Liu, Tianjin.,Cen, Jin.,Chen, Xiaotao.,Cheng, Xin.,Hui, Lijian.,...&,.(2017).Lineage conversion of mouse fibroblasts to pancreatic alpha-cells.EXPERIMENTAL AND MOLECULAR MEDICINE,49(-),e350. |
| MLA | Liu, Tianjin,et al."Lineage conversion of mouse fibroblasts to pancreatic alpha-cells".EXPERIMENTAL AND MOLECULAR MEDICINE 49.-(2017):e350. |
入库方式： OAI收割
来源：上海营养与健康研究所
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