The transcriptome difference between colorectal tumor and normal tissues revealed by single-cell sequencing
文献类型：期刊论文
| 作者 | Zhang, Guo-Liang1; Pan, Le-Lin1; Wang, Jin-Hai1; Huang, Tao2; , |
| 刊名 | JOURNAL OF CANCER
 |
| 出版日期 | 2019 |
| 卷号 | 10期号:23页码:5883-5890 |
| 关键词 | colorectal cancer single-cell sequencing transcriptome support vector machine minimal redundancy maximal relevance incremental feature selection |
| ISSN号 | 1837-9664 |
| DOI | 10.7150/jca.32267 |
| 文献子类 | Article |
| 英文摘要 | The previous cancer studies were difficult to reproduce since the tumor tissues were analyzed directly. But the tumor tissues were actually a mixture of different cancer cells. The transcriptome of single-cell was much robust than the transcriptome of a mixed tissue. The single-cell transcriptome had much smaller variance. In this study, we analyzed the single-cell transcriptome of 272 colorectal cancer (CRC) epithelial cells and 160 normal epithelial cells and identified 342 discriminative transcripts using advanced machine learning methods. The most discriminative transcripts were LGALS4, PHGR1, C15orf48, HEPACAM2, PERP, FABP1, FCGBP, MT1G, TSPAN1 and CKB. We further clustered the 342 transcripts into two categories. The upregulated transcripts in CRC epithelial cells were significantly enriched in Ribosome, Protein processing in endoplasmic reticulum, Antigen processing and presentation and p53 signaling pathway. The downregulated transcripts in CRC epithelial cells were significantly enriched in Mineral absorption, Aldosterone-regulated sodium reabsorption and Oxidative phosphorylation pathways. The biological analysis of the discriminative transcripts revealed the possible mechanism of colorectal cancer. |
| 学科主题 | Oncology |
| WOS关键词 | CANCER CELLS ; EXPRESSION ; IDENTIFICATION ; PROLIFERATION ; EPITHELIUM ; RELEVANCE ; CARCINOMA ; VARIANT |
| 语种 | 英语 |
| CSCD记录号 | CSCD:31737124 |
| WOS记录号 | WOS:000490321900030 |
| 出版者 | IVYSPRING INT PUBL |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/1216]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所 |
| 作者单位 | 1.Zhejiang Univ, Affiliated Hosp 1, Coll Med, Dept Colorectal Surg, Hangzhou 310003, Zhejiang, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Nutr & Hlth, Shanghai 200031, Peoples R China, |
| 推荐引用方式 GB/T 7714 | Zhang, Guo-Liang,Pan, Le-Lin,Wang, Jin-Hai,et al. The transcriptome difference between colorectal tumor and normal tissues revealed by single-cell sequencing[J]. JOURNAL OF CANCER,2019,10(23):5883-5890. |
| APA | Zhang, Guo-Liang,Pan, Le-Lin,Wang, Jin-Hai,Huang, Tao,&,.(2019).The transcriptome difference between colorectal tumor and normal tissues revealed by single-cell sequencing.JOURNAL OF CANCER,10(23),5883-5890. |
| MLA | Zhang, Guo-Liang,et al."The transcriptome difference between colorectal tumor and normal tissues revealed by single-cell sequencing".JOURNAL OF CANCER 10.23(2019):5883-5890. |
入库方式： OAI收割
来源：上海营养与健康研究所
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