Estradiol inhibits fMLP-induced neutrophil migration and superoxide production by upregulating MKP-2 and dephosphorylating ERK
文献类型：期刊论文
| 作者 | Zhang, Ping1; Wan, Dapeng1; Su, Hao1; Wang, Zhaodong1; Hou, Ruixing1,4; Fu, Yi2; Rui, Huajuan3; Ju, Jihui4; Jin, Qianheng4; Le, Yingying5 |
| 刊名 | INTERNATIONAL IMMUNOPHARMACOLOGY
 |
| 出版日期 | 2019 |
| 卷号 | 75期号:-页码:105787 |
| 关键词 | Estradiol Neutrophil Chemotaxis Reactive oxygen species MKP-2 ERK |
| ISSN号 | 1567-5769 |
| DOI | 10.1016/j.intimp.2019.105787 |
| 文献子类 | Article |
| 英文摘要 | Estrogen has been reported to inhibit neutrophil infiltration related inflammation and suppress neutrophils migration in vitro, but the underlying mechanism is not fully understood. By using HL-60 differentiated neutrophil-like cells (dHL-60) and human neutrophils, we examined the effect of 17-beta estradiol (E-2) on cell migration and superoxide production in response to chemotactic peptide N-formyl-methionyl-leucyl-phenylalanine (fMLP) and explored the mechanisms involved. We found that fMLP significantly induced dHL-60 cell and neutrophil migration and superoxide production, which was inhibited by ERK inhibitor PD98059. E-2 significantly inhibited fMLP-induced dHL-60 cell and neutrophil migration and superoxide production at both physiological and pharmacological concentrations. Mechanistic studies showed that pretreatment of these cells with E-2 rapidly elevated the protein level of mitogen-activated protein kinase phosphatase 2 (MKP-2) and inhibited fMLP-induced ERK phosphorylation. Pretreatment of these cells with estrogen receptor (ER) antagonist ICI 182780 reversed the inhibition of fMP-induced cell migration and superoxide production, and the induction of MKP-2 expression and the suppression of fMP-induced ERK phosphorylation by E-2. However, pretreatment of cells with G-protein coupled ER antagonist G15 had no such effect. Collectively, these results demonstrate that fMLP stimulates neutrophil chemotaxis and superoxide production through activating ERK, and indicate that ER-mediated upregulation of MKP-2 may dephosphorylate ERK and contribute to the inhibitory effect of E-2 on neutrophil activation by fMLP. Our study reveals new mechanisms involved in the anti-inflammatory activity of estrogen. |
| 学科主题 | Biotechnology & Applied Microbiology ; Oncology |
| WOS关键词 | ACTIVATED PROTEIN-KINASE ; ISCHEMIA-REPERFUSION ; POSTTRANSLATIONAL REGULATION ; PHOSPHATASE-2 MKP-2 ; OXIDATIVE BURST ; ESTROGEN ACTION ; SEX-HORMONES ; HL-60 CELLS ; TNF-ALPHA ; RECEPTOR |
| 语种 | 英语 |
| CSCD记录号 | CSCD:31401382 |
| WOS记录号 | WOS:000488998800063 |
| 出版者 | ELSEVIER |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/1217]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所 |
| 作者单位 | 1.Soochow Univ, Ruihua Affiliated Hosp, Inst Hand Surg, Suzhou 215100, Jiangsu, Peoples R China; 2.Soochow Univ, Sch Biol & Basic Med Sci, Dept Human Anat Histol & Embryol, Suzhou 215007, Jiangsu, Peoples R China; 3.Soochow Univ, Ruihua Affiliated Hosp, Dept Clin Lab, Suzhou 215100, Jiangsu, Peoples R China; 4.Soochow Univ, Ruihua Affiliated Hosp, Dept Hand Surg, Suzhou 215100, Jiangsu, Peoples R China; 5.Univ Chinese Acad Sci, Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Nutr & Hlth,CAS Key Lab Nutr Metab, Shanghai, Peoples R China, |
| 推荐引用方式 GB/T 7714 | Zhang, Ping,Wan, Dapeng,Su, Hao,et al. Estradiol inhibits fMLP-induced neutrophil migration and superoxide production by upregulating MKP-2 and dephosphorylating ERK[J]. INTERNATIONAL IMMUNOPHARMACOLOGY,2019,75(-):105787. |
| APA | Zhang, Ping.,Wan, Dapeng.,Su, Hao.,Wang, Zhaodong.,Hou, Ruixing.,...&,.(2019).Estradiol inhibits fMLP-induced neutrophil migration and superoxide production by upregulating MKP-2 and dephosphorylating ERK.INTERNATIONAL IMMUNOPHARMACOLOGY,75(-),105787. |
| MLA | Zhang, Ping,et al."Estradiol inhibits fMLP-induced neutrophil migration and superoxide production by upregulating MKP-2 and dephosphorylating ERK".INTERNATIONAL IMMUNOPHARMACOLOGY 75.-(2019):105787. |
入库方式： OAI收割
来源：上海营养与健康研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。