Nanodiamonds Mediate Oral Delivery of Proteins for Stem Cell Activation and Intestinal Remodeling in Drosophila
文献类型：期刊论文
| 作者 | Hu, Xingjie1,2; Yin, Min1,2; Wang, Lihua1,2; Chen, Nan1,2; Fan, Chunhai1,2; Li, Xiaojiao3,4; Li, Ping3,4; Huang, Ping3,4; Wang, Hui3,4; Song, Haiyun3,4 |
| 刊名 | ACS APPLIED MATERIALS & INTERFACES
 |
| 出版日期 | 2017 |
| 卷号 | 9期号:22页码:18575-18583 |
| 关键词 | nanodiamonds protein delivery oral delivery stem cells microenvironment intestinal remodeling |
| ISSN号 | 1944-8244 |
| DOI | 10.1021/acsami.7b04788 |
| 文献子类 | Article |
| 英文摘要 | Introduction of exogenous biomacromolecules into living systems is of great interest in genome editing, cancer immunotherapy, and stem cell reprogramming. Whereas current strategies generally depend on nucleic acids transfection, direct delivery of functional proteins that provides enhanced specificity, increased safety, and fast and temporal regulation is highly desirable. Nevertheless, intracellular delivery of intact and bioactive proteins, especially in vivo, remains poorly explored. In this study, we developed a nanodiamonds (NDs)-based protein delivery system in cultured cells and in Drosophila that showed high adsorption, high efficiency, and effective cytosolic release of fully functional proteins. Through live-cell imaging, we observed a novel phenomenon wherein a substantial amount of internalized NDsprotein complex rejected fusion with the early endosome, thereby evading protein degradation in the lysosome. More significantly, we demonstrated that dietary NDsRNase induced apoptosis in enterocytes, stimulating regenerative divisions in intestinal stem cells and increasing the number of stem cells and precursor cells in Drosophila intestine. As stem cells are poorly accessible by exogenous agents in vivo, NDs-mediated oral delivery of proteins provides a new approach to modulate the stem cell microenvironment for intestinal remodeling, which has important implications for colorectal cancer therapy and regenerative medicine. |
| 学科主题 | Science & Technology - Other Topics ; Materials Science |
| WOS关键词 | WALLED CARBON NANOTUBES ; FLUORESCENT NANODIAMONDS ; DNA NANOSTRUCTURES ; DRUG-DELIVERY ; NANOPARTICLES ; CANCER ; PATHWAY ; AUTOPHAGY ; OXIDE ; BIOCOMPATIBILITY |
| 语种 | 英语 |
| WOS记录号 | WOS:000403136400022 |
| 出版者 | AMER CHEMICAL SOC |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/1223]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所 |
| 作者单位 | 1.Univ Chinese Acad Sci, Chinese Acad Sci, Shanghai Inst Appl Phys, Div Phys Biol, Shanghai 201800, Peoples R China; 2.Univ Chinese Acad Sci, Chinese Acad Sci, Shanghai Inst Appl Phys,CAS Key Lab Interfacial P, Bioimaging Ctr,Shanghai Synchrotron Radiat Facil, Shanghai 201800, Peoples R China; 3.Univ Chinese Acad Sci, Chinese Acad Sci, Key Lab food safety Res, Inst Nutr Sci,Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China; 4.Minist Hlth, Key Lab food safety risk Assessment, Beijing 100021, Peoples R China; 5.Guangzhou Med Univ, Sch Publ Hlth, Guangzhou 511436, Guangdong, Peoples R China, |
| 推荐引用方式 GB/T 7714 | Hu, Xingjie,Yin, Min,Wang, Lihua,et al. Nanodiamonds Mediate Oral Delivery of Proteins for Stem Cell Activation and Intestinal Remodeling in Drosophila[J]. ACS APPLIED MATERIALS & INTERFACES,2017,9(22):18575-18583. |
| APA | Hu, Xingjie.,Yin, Min.,Wang, Lihua.,Chen, Nan.,Fan, Chunhai.,...&,.(2017).Nanodiamonds Mediate Oral Delivery of Proteins for Stem Cell Activation and Intestinal Remodeling in Drosophila.ACS APPLIED MATERIALS & INTERFACES,9(22),18575-18583. |
| MLA | Hu, Xingjie,et al."Nanodiamonds Mediate Oral Delivery of Proteins for Stem Cell Activation and Intestinal Remodeling in Drosophila".ACS APPLIED MATERIALS & INTERFACES 9.22(2017):18575-18583. |
入库方式： OAI收割
来源：上海营养与健康研究所
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