miR-212-5p suppresses lipid accumulation by targeting FAS and SCD1
文献类型：期刊论文
| 作者 | Guo, Yajie; Yu, Junjie; Wang, Chunxia; Li, Kai; Liu, Bin; Du, Ying; Xiao, Fei; Chen, Shanghai; Guo, Feifan; , |
| 刊名 | JOURNAL OF MOLECULAR ENDOCRINOLOGY
 |
| 出版日期 | 2017 |
| 卷号 | 59期号:3页码:205-217 |
| 关键词 | miR-212-5p FAS SCD1 lipid accumulation NAFLD |
| ISSN号 | 0952-5041 |
| DOI | 10.1530/JME-16-0179 |
| 文献子类 | Article |
| 英文摘要 | MicroRNAs, a class of small noncoding RNAs, are implicated in controlling a variety of biological processes. We have shown that leucine deprivation suppresses lipogenesis by inhibiting fatty acid synthase (FAS) expression in the liver previously; the aim of our current study is to investigate which kind of microRNA is involved in the regulation of FAS expression in response to leucine deprivation. Here, we indicated that microRNA212-5p specifically binds to mouse FAS 3' UTR and inhibits its activity. Leucine deficiency significantly increased the mRNA levels of miR-212-5p in the livers of mice. Further studies proved that miR-212-5p also directly binds to the 3' UTR of stearoyl-CoA desaturase-1 (SCD1) to inhibit its activity. Overexpression of miR-212-5p decreases the protein levels of FAS and SCD1 in vitro and in vivo, and silencing of miR-212-5p has the opposite effects in mouse primary hepatocytes. Moreover, overexpression of miR-212-5p significantly decreases triglyceride (TG) accumulation in primary hepatocytes and in the livers of mice injected with adenovirus-mediated overexpressing of miR-212-5p (Ad-miR-212). Interestingly, inhibition of miR-212-5p reverses the suppressive effects of leucine deficiency on FAS and SCD1 expression, as well as TG accumulation in mouse primary hepatocytes. Finally, we demonstrate that leucine deficiency induces the expression of miR-212-5p in a GCN2/ATF4-dependent manner. Taken together, our results demonstrate a novel function of hepatic miR-212-5p in the regulation of lipid metabolism which represents a potential therapeutic target for the treatment of non-alcohol fatty liver diseases (NAFLD). |
| 学科主题 | Endocrinology & Metabolism |
| WOS关键词 | FATTY LIVER-DISEASE ; DE-NOVO LIPOGENESIS ; INSULIN-RESISTANCE ; HEPATIC STEATOSIS ; AMINO-ACID ; MICRORNA EXPRESSION ; METABOLIC SYNDROME ; GCN2/ATF4 PATHWAY ; BINDING-PROTEIN ; X RECEPTOR |
| 语种 | 英语 |
| WOS记录号 | WOS:000417615000004 |
| 出版者 | BIOSCIENTIFICA LTD |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/1225]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所 |
| 作者单位 | Univ Chinese Acad Sci, Chinese Acad Sci, Inst Nutr Sci, Key Lab Nutr & Metab,Shanghai Inst Biol Sci, Shanghai, Peoples R China, |
| 推荐引用方式 GB/T 7714 | Guo, Yajie,Yu, Junjie,Wang, Chunxia,et al. miR-212-5p suppresses lipid accumulation by targeting FAS and SCD1[J]. JOURNAL OF MOLECULAR ENDOCRINOLOGY,2017,59(3):205-217. |
| APA | Guo, Yajie.,Yu, Junjie.,Wang, Chunxia.,Li, Kai.,Liu, Bin.,...&,.(2017).miR-212-5p suppresses lipid accumulation by targeting FAS and SCD1.JOURNAL OF MOLECULAR ENDOCRINOLOGY,59(3),205-217. |
| MLA | Guo, Yajie,et al."miR-212-5p suppresses lipid accumulation by targeting FAS and SCD1".JOURNAL OF MOLECULAR ENDOCRINOLOGY 59.3(2017):205-217. |
入库方式： OAI收割
来源：上海营养与健康研究所
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