Single-cell analysis reveals lineage segregation in early post-implantation mouse embryos
文献类型：期刊论文
| 作者 | Wen, Jing1; Fang, Zhuoqing1; Jin, Ying1,2; Zeng, Yanwu2; Gu, Junjie2; Ge, Laixiang2; Tang, Fan2; Qu, Zepeng2; Hu, Jing2; Cui, Yaru3 |
| 刊名 | JOURNAL OF BIOLOGICAL CHEMISTRY
 |
| 出版日期 | 2017 |
| 卷号 | 292期号:23页码:9840-9854 |
| ISSN号 | 0021-9258 |
| 关键词 | Tibetan adaptive genetic variant high-altitude adaptation next-generation sequencing (NGS) archaic ancestry expression quantitative traits loci (eQTL) tissue-specific expression hemoglobin concentration hypoxia |
| DOI | 10.1074/jbc.M117.780585 |
| 文献子类 | Article |
| 英文摘要 | The mammalian post-implantation embryo has been extensively investigated at the tissue level. However, to unravel the molecular basis for the cell-fate plasticity and determination, it is essential to study the characteristics of individual cells. In particular, the individual definitive endoderm (DE) cells have not been characterized in vivo. Here, we report gene expression patterns in single cells freshly isolated from mouse embryos on days 5.5 and 6.5. Initial transcriptome data from 124 single cells yielded signature genes for the epiblast, visceral endoderm, and extra-embryonic ectoderm and revealed a unique distribution pattern of fibroblast growth factor (FGF) ligands and receptors. Further analysis indicated that early-stage epiblast cells do not segregate into lineages of the major germ layers. Instead, some cells began to diverge from epiblast cells, displaying molecular features of the premesendoderm by expressing higher levels of mesendoderm markers and lower levels of Sox3 transcripts. Analysis of single-cell high-throughput quantitative RT-PCR data from 441 cells identified a late stage of the day 6.5 embryo in which mesoderm and DE cells emerge, with many of them coexpressing Oct4 and Gata6. Analysis of single-cell RNA-sequence data from 112 cells of the late-stage day 6.5 embryos revealed differentially expressed signaling genes and networks of transcription factors that might underlie the segregation of the mesoderm and DE lineages. Moreover, we discovered a subpopulation of mesoderm cells that possess molecular features of the extraembryonic mesoderm. This study provides fundamental insight into the molecular basis for lineage segregation in post-implantation mouse embryos. |
| 学科主题 | Biochemistry & Molecular Biology |
| WOS关键词 | SET ENRICHMENT ANALYSIS ; STEM-CELLS ; TRANSCRIPTION FACTOR ; FATE DECISIONS ; EXPRESSION ANALYSIS ; PROGENITOR CELLS ; BETA-CATENIN ; GASTRULATION ; ENDODERM ; EPIBLAST |
| 语种 | 英语 |
| 出版者 | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC |
| WOS记录号 | WOS:000403113000031 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/1258]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所 |
| 作者单位 | 1.Shanghai Jiao Tong Univ, Sch Med,Shanghai Inst Biol Sci, Chinese Acad Sci,Key Lab Stem Cell Biol, CAS Ctr Excellence Mol Cell Sci,Inst Hlth Sci, Shanghai 200031, Peoples R China; 2.Shanghai Jiao Tong Univ, Dept Mol Dev Biol, Sch Med, Shanghai 200025, Peoples R China; 3.Tongji Univ, Sch Med, Tongji Hosp, Stem Cell Translat Res Ctr, Shanghai 200092, Peoples R China, |
| 推荐引用方式 GB/T 7714 | Wen, Jing,Fang, Zhuoqing,Jin, Ying,et al. Single-cell analysis reveals lineage segregation in early post-implantation mouse embryos[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2017,292(23):9840-9854. |
| APA | Wen, Jing.,Fang, Zhuoqing.,Jin, Ying.,Zeng, Yanwu.,Gu, Junjie.,...&,.(2017).Single-cell analysis reveals lineage segregation in early post-implantation mouse embryos.JOURNAL OF BIOLOGICAL CHEMISTRY,292(23),9840-9854. |
| MLA | Wen, Jing,et al."Single-cell analysis reveals lineage segregation in early post-implantation mouse embryos".JOURNAL OF BIOLOGICAL CHEMISTRY 292.23(2017):9840-9854. |
入库方式： OAI收割
来源：上海营养与健康研究所
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