SPSB1-mediated HnRNP A1 ubiquitylation regulates alternative splicing and cell migration in EGF signaling
文献类型：期刊论文
| 作者 | Wang, Feng1,2; Li, Na1,2; Zhu, Hong1,2; Jia, Ting-Ting1,2; Hui, Jingyi1,2; Fu, Xing3; Chen, Peng4; Ji, Hongbin4; Hu, Ronggui4; Wu, Ping5,6 |
| 刊名 | CELL RESEARCH
 |
| 出版日期 | 2017 |
| 卷号 | 27期号:4页码:540-558 |
| 关键词 | alternative splicing EGF signaling hnRNP A1 protein ubiquitylation Rac1 SPSB1 |
| ISSN号 | 1001-0602 |
| DOI | 10.1038/cr.2017.7 |
| 文献子类 | Article |
| 英文摘要 | Extracellular signals have been shown to impact on alternative pre-mRNA splicing; however, the molecular mechanisms and biological significance of signal-induced splicing regulation remain largely unknown. Here, we report that epidermal growth factor (EGF) induces splicing changes through ubiquitylation of a well-known splicing regulator, hnRNP A1. EGF signaling upregulates an E3 ubiquitin (Ub) ligase adaptor, SPRY domain-containing SOCS box protein 1 (SPSB1), which recruits Elongin B/C-Cullin complexes to conjugate lysine 29-linked polyUb chains onto hnRNP A1. Importantly, SPSB1 and ubiquitylation of hnRNP A1 have a critical role in EGF-driven cell migration. Mechanistically, EGF-induced ubiquitylation of hnRNP A1 together with the activation of SR protein kinases (SRPKs) results in the upregulation of a Rac1 splicing isoform, Rac1b, to promote cell motility. These findings unravel a novel crosstalk between protein ubiquitylation and alternative splicing in EGF/EGF receptor signaling, and identify a new EGF/SPSB1/hnRNP A1/Rac1 axis in modulating cell migration, which may have important implications for cancer treatment. |
| 学科主题 | Cell Biology |
| WOS关键词 | EPIDERMAL-GROWTH-FACTOR ; RNA-BINDING-PROTEINS ; SOCS-BOX MOTIF ; MESSENGER-RNA ; NUCLEAR EXPORT ; BREAST-CANCER ; PROTEASOMAL DEGRADATION ; GLOBAL ANALYSIS ; FUNCTIONAL-ROLE ; SR PROTEINS |
| 语种 | 英语 |
| CSCD记录号 | CSCD:31664040 |
| WOS记录号 | WOS:000398770500009 |
| 出版者 | INST BIOCHEMISTRY & CELL BIOLOGY |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/1267]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所 |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, CAS Ctr Excellence Mol Cell Sci, State Key Lab Mol Biol, Shanghai 200031, Peoples R China; 2.Univ Chinese Acad Sci, Shanghai 200031, Peoples R China; 3.Chinese Acad Sci, Shanghai Ctr Plant Stress Biol, Shanghai 201602, Peoples R China; 4.Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, CAS Ctr Excellence Mol Sci, Innovat Ctr Cell Signaling Network,Key Lab Syst B, Shanghai 200031, Peoples R China; 5.Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, Natl Ctr Prot Sci Shanghai, Shanghai 200031, Peoples R China; 6.Chinese Acad Sci, Shanghai Sci Res Ctr, Shanghai 201204, Peoples R China; 7.Chinese Acad Sci, CAS MPG Partner Inst Computat Biol, Key Lab Computat Biol, CAS Ctr Excellence Mol Cell Sci, Shanghai 200031, Peoples R China, |
| 推荐引用方式 GB/T 7714 | Wang, Feng,Li, Na,Zhu, Hong,et al. SPSB1-mediated HnRNP A1 ubiquitylation regulates alternative splicing and cell migration in EGF signaling[J]. CELL RESEARCH,2017,27(4):540-558. |
| APA | Wang, Feng.,Li, Na.,Zhu, Hong.,Jia, Ting-Ting.,Hui, Jingyi.,...&,.(2017).SPSB1-mediated HnRNP A1 ubiquitylation regulates alternative splicing and cell migration in EGF signaling.CELL RESEARCH,27(4),540-558. |
| MLA | Wang, Feng,et al."SPSB1-mediated HnRNP A1 ubiquitylation regulates alternative splicing and cell migration in EGF signaling".CELL RESEARCH 27.4(2017):540-558. |
入库方式： OAI收割
来源：上海营养与健康研究所
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