The Output of Protein-Coding Genes Shifts to Circular RNAs When the Pre-mRNA Processing Machinery Is Limiting
文献类型：期刊论文
| 作者 | Liang, Dongming1; Tatomer, Deirdre C.1; Wilusz, Jeremy E.1; Luo, Zheng2; Yang, Li2; Wu, Huang3; Chen, Ling-Ling3,4; Yang, Li4; Cherry, Sara5; , |
| 刊名 | MOLECULAR CELL
 |
| 出版日期 | 2017 |
| 卷号 | 68期号:5页码:940-+ |
| 关键词 | developmental biology disease model regeneration zebrafish |
| ISSN号 | 1097-2765 |
| DOI | 10.1016/j.molcel.2017.10.034 |
| 文献子类 | Article |
| 英文摘要 | Many eukaryotic genes generate linear mRNAs and circular RNAs, but it is largely unknown how the ratio of linear to circular RNA is controlled or modulated. Using RNAi screening in Drosophila cells, we identify many core spliceosome and transcription termination factors that control the RNA outputs of reporter and endogenous genes. When spliceosome components were depleted or inhibited pharmacologically, the steady-state levels of circular RNAs increased while expression of their associated linear mRNAs concomitantly decreased. Upon inhibiting RNA polymerase II termination via depletion of the cleavage/polyadenylation machinery, circular RNA levels were similarly increased. This is because readthrough transcripts now extend into downstream genes and are subjected to backsplicing. In total, these results demonstrate that inhibition or slowing of canonical pre-mRNA processing events shifts the steady-state output of protein-coding genes toward circular RNAs. This is in part because nascent RNAs become directed into alternative pathways that lead to circular RNA production. |
| 学科主题 | Biochemistry & Molecular Biology ; Cell Biology |
| WOS关键词 | CORE SPLICEOSOMAL MACHINERY ; EXON CIRCULARIZATION ; COMBINATORIAL CONTROL ; BINDING PROTEINS ; POLYMERASE-II ; HUMAN GENOME ; HUMAN-CELLS ; TRANSCRIPTION ; DROSOPHILA ; BIOGENESIS |
| 语种 | 英语 |
| CSCD记录号 | CSCD:30481142 |
| WOS记录号 | WOS:000417646500013 |
| 出版者 | CELL PRESS |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/1293]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所 |
| 作者单位 | 1.Univ Penn, Perelman Sch Med, Dept Biochem & Biophys, Philadelphia, PA 19104 USA; 2.Chinese Acad Sci, Univ Chinese Acad Sci, Key Lab Computat Biol, CAS MPG Partner Inst Computat Biol,Shanghai Inst, Shanghai 200031, Peoples R China; 3.Chinese Acad Sci, Univ Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, State Key Lab Mol Biol,CAS Ctr Excellence Mol Cel, Shanghai 200031, Peoples R China; 4.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China; 5.Univ Penn, Perelman Sch Med, Dept Microbiol, Philadelphia, PA 19104 USA, |
| 推荐引用方式 GB/T 7714 | Liang, Dongming,Tatomer, Deirdre C.,Wilusz, Jeremy E.,et al. The Output of Protein-Coding Genes Shifts to Circular RNAs When the Pre-mRNA Processing Machinery Is Limiting[J]. MOLECULAR CELL,2017,68(5):940-+. |
| APA | Liang, Dongming.,Tatomer, Deirdre C..,Wilusz, Jeremy E..,Luo, Zheng.,Yang, Li.,...&,.(2017).The Output of Protein-Coding Genes Shifts to Circular RNAs When the Pre-mRNA Processing Machinery Is Limiting.MOLECULAR CELL,68(5),940-+. |
| MLA | Liang, Dongming,et al."The Output of Protein-Coding Genes Shifts to Circular RNAs When the Pre-mRNA Processing Machinery Is Limiting".MOLECULAR CELL 68.5(2017):940-+. |
入库方式： OAI收割
来源：上海营养与健康研究所
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